气道上皮细胞紧密连接在小鼠哮喘中的病理学改变  

The pathological changes in airway epitheilal tight junctions in mouse model with asthma

作　　者：李凡[1] 吴琦[2] 孙昕[2] 李宽[2] 张颖超[3] 李雪[2] 李玉[2] 张秋阳[2] 徐龙[2] 陈怀永[2] 

机构地区：[1]天津医科大学,300070 [2]天津市海河医院基础医学实验部 [3]天津市宝坻区人民医院呼吸科

出　　处：《天津医药》2016年第1期23-25,129,共4页Tianjin Medical Journal

基　　金：国家自然科学基金面上项目(31471121);天津市科委基础项目(14JCYBJC25700;13JCYBJC40000)

摘　　要：目的探讨小鼠支气管哮喘(哮喘)中气道上皮紧密连接的病理学改变。方法 10只小鼠随机分为对照组和哮喘组,每组5只。哮喘组于第0、7天腹腔注射鸡卵清蛋白(OVA)溶液,在第14、15、16天以雾化的OVA熏诱小鼠,1 h/次、1次/d;对照组用PBS溶液替代OVA;2组小鼠在第18天回收肺组织,采用免疫荧光染色法评价小鼠哮喘模型的有效性,荧光定量PCR检测紧密连接相关蛋白基因Claudin 1、Claudin 3、Claudin 4、Claudin 5、Claudin 7、Clau-din 10在肺组织中的表达。结果哮喘组Claudin 3、Claudin 4、Claudin 10 m RNA水平较对照组低(P<0.05),而Clau-din 1、Claudin 5、Claudin 7 m RNA水平与对照组比较差异无统计学意义(P>0.05)。结论哮喘病理过程中气道上皮细胞紧密连接松散,提示气道上皮屏障破坏。Objective To investigate the pathological changes of airway epithelium mucosa in asthmatic mice. Meth- ods Ten mice were divided into two groups： control group and asthma group, five mice in each group. Asthma group was sensitized with chicken ovalbumin （OVA） by intraperitoneal injection on day 0 and 7. Mice were then challenged with OVA on day 14, 15, and 16, 1 hour each time, once a day. Control group was given PBS solution instead of OVA. Lungs were col- lected at day 18 in two groups. Immunofluorescence staining was used to evaluate asthmatic mouse model. Quantitative PCR was applied to detect the expression of Claudin 1, Claudin 3, Claudin 4, Claudin 5, Claudin 7 and Claudin 10 in lung tissues. Results The expressions of Claudin 3, Claudin 4 and Claudin 10 were significantly lower in asthma group than those in control group （P 〈 0.05）. There were no significant differences in Claudin 1, Claudin 5 and Claudin 7 mRNA levels between control group and asthma group （P 〉 0.05）. Conclusion Tight junctions of airway epithelium are loosed in asthma, suggest- ing that epithelial permeability is increased.

关 键 词：哮喘 连接蛋白类 气道上皮黏膜 钙激活氯离子通道蛋白3 紧密连接蛋白 CLAUDIN 3 

分 类 号：R562.25[医药卫生—呼吸系统]