异位内膜氧化应激及雌激素受体水平与盆腔痛关系的研究  

The relationship between oxidative stress and estrogen receptor levels in ectopic endometrium and pelvic pain

作　　者：郭蕊萌[1] 尹利荣[1] 

机构地区：[1]天津医科大学第二医院妇科,300211

出　　处：《天津医药》2016年第1期88-90,130,共4页Tianjin Medical Journal

摘　　要：目的探讨异位内膜氧化应激(OS)及雌激素受体(ER)水平与盆腔痛的关系。方法取轻、中、重度盆腔痛子宫内膜异位症(EMs)患者异位内膜作为轻度组(29例)、中度组(34例)及重度组(26例),取正常子宫内膜作为对照组(30例)。采用黄嘌呤氧化酶法检测和对比各组内膜中超氧化物歧化酶(SOD)水平;采用硫代巴比妥酸比色法检测和对比各组内膜中丙二醛(MDA)水平;采用免疫组化法检测和对比各组内膜中ER水平;分析3组异位内膜中MDA、SOD与ER水平相关性。结果 3组异位内膜中SOD水平均低于对照组,MDA及ER水平均高于对照组(均P<0.05);3组异位内膜中,随疼痛程度增加,SOD水平逐渐降低,MDA及ER水平逐渐升高(均P<0.05);轻度组内膜中MDA水平与ER水平无相关性,中度及重度组内膜中MDA水平与ER水平呈正相关(P<0.05),3组异位内膜中SOD水平与ER水平均无相关性。结论异位内膜存在OS及ER过表达,二者参与EMs盆腔痛的发生并发挥协同作用。Objective To discuss the role of oxidative stress （OS） and estrogen receptor （ER） levels in ectopic endome- trium in patients with pelvic pain. Methods The ectopic endometrium was taken from patients with mild, moderate and se- vere pelvic pain, and samples were divided into mild group （n=29）, moderate group （n=34） and severe group （n=26）. The nor- mal samples of endometrium （n=30） were used as control group. Xanthine oxidase method was used to detect the level of su- peroxide dismutase （SOD）, thiobarbituric acid colorimetric method was used to detect the level of malondialdehyde （MDA） and immunohistochemistry was used to detect the level of estrogen receptor （ER）. The correlation of MDA, SOD and ER lev- els was analysed between three ectopic endometrium groups. Results The SOD levels were significantly lower and MDA and ER levels were significantly higher in three ectopic endometrium groups than those in control group （P 〈0.05）. The SOD levels were decreased, while MDA and ER levels were gradually increased with increased pain intensity in three groups of ec- topic endometrium （P 〈 0.05）. There was no correlation between MDA level and ER level in mild group （P 〉 0.05）. A posi- tive correlation was found between MDA and ER levels in moderate and severe groups （P 〈 0.05）. There was no relationship between SOD and ER levels in three ectopic endometrium groups （P 〉 0.05）. Conclusion Over-expression of OS and ER presents in ectopic endometrium, and both participate in the occurrence of pelvic pain, and create synergies in pelvic pain of ectopie endometrium.

关 键 词：子宫内膜异位症 氧化性应激 受体 雌激素 超氧化物歧化酶 丙二醛 盆腔痛 

分 类 号：R711.71[医药卫生—妇产科学]