糖尿病合并梅毒兔模型的建立及其肾损伤的研究  

Establishment of a rabbit model of diabetes mellitus in combination with syphilis and analysis of kidney damages

作　　者：刘双全[1] 刘炀[1] 朱雅玲[1] 曾丹[1] 易婷[1] 罗韬[1] 谢小平[1] 吴移谋[2] 

机构地区：[1]南华大学附属第一医院检验科,衡阳421001 [2]南华大学病原生物学研究所,衡阳421001

出　　处：《中华微生物学和免疫学杂志》2015年第12期921-925,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81201331）

摘　　要：目的构建糖尿病合并梅毒兔感染模型，动态观察肾脏损伤情况及初步探讨其机制。方法采用四氧嘧啶诱导法构建单独糖尿病组（DM组），采用四氧嘧啶诱导糖尿病再接种梅毒螺旋体致其感染构建合并梅毒组（H组），另设单独梅毒组（T组）和正常对照组（C组），动态观察各组兔肾脏损伤改变情况并初步研究炎症相关硫氧还蛋白相互作用蛋白（thioredoxin-interacting protein，TXNIP）的表达情况。结果成功构建了各组兔感染模型，自第8周起，与T组和DM组相比，H组兔随实验时间增长尿素氮及肌酐明显升高；与C组比较，各实验组肾脏组织有明显损伤，且H组损伤程度明显比单独疾病组T组和DM组严重；免疫组化结果显示，各实验组肾脏TXNIP表达均增加，但合并感染H组TXNIP表达量明显高于单独感染组T组和DM组。结论糖尿病合并梅毒兔模型能引起比单独疾病组更严重的肾脏损伤，其损伤机制可能与TXNIP的高表达有关。Objective To establish a rabbit model of diabetes mellitus in combination with syphilis for further monitoring the dynamic changes in rabbits＇ kidneys and investigating the possible pathogenic mechanism. Methods Twenty-four male rabbits were divided into four groups including diabetes group （group DM, n = 8）, syphilis group （group T, n = 5 ）, diabetes mellitus in combination with syphilis group （ group H, n = 8） and control group （group C, n = 3）. The rabbit models for groups DM, T and H were respectively established by injecting rabbits with alloxan, Treponema pallidum strains and alloxan in combination with Treponema pallidum strains. Kidney damages in each rabbit were dynamically monitored. Immunohistochemical staining was used to measure the expression of an inflammation related protein, thioredoxin-interacting protein （TXNIP）, in kidney tissues of each rabbit. Results The rabbit models for three experimental groups were successfully established. Compared with the rabbits form groups T and DM, the rabbits from group H showed higher levels of blood urea nitrogen （BUN） and creatinine （Cr） since the eighth week after modeling. Compared with the rabbits form group C, those from the three experimental groups showed obvious kidney damages and the most severe damages in kidney tissues were observed in rabbits from group H. Results of the immunohistochemical staining showed that the expression of TXNIP in kidney tissues were enhanced in rabbits from the three experimental groups, especially in those from the group H. Conclusion Diabetes mellitus in combination with syphilis could induce more severe kidney damages in rabbits than those induced by diabetes or syphilis alone. The possible mechanism might relate to the hyper-expression of TXNIP in kidney tissues.

关 键 词：糖尿病 梅毒 兔模型 肾损伤 TXNIP 

分 类 号：R587.2[医药卫生—内分泌] R759.1[医药卫生—内科学] R-332[医药卫生—临床医学]