加味二陈汤对ApoE^(-/-)小鼠炎症标志物IL-6和TNF-α的调节作用  

The Effects of Modified “Erchen Decoction”on the Expression of Inflammatory Marker IL-6,TNF-α on Apo E^(-/-) Mice

作　　者：谢雪姣[1] 李亚梅[1] 徐佳[1] 廖端芳[1] 吴若霞[1] 尹栋梁[1] 陈聪伶 

机构地区：[1]湖南中医药大学仲景学说教研室,湖南长沙410208

出　　处：《中南医学科学杂志》2015年第6期605-609,共5页Medical Science Journal of Central South China

基　　金：全国第五批老中医药专家学术经验继承项目;湖南省教育厅科研青年项目(编号11B092);湖南省中医药科研计划项目(编号201236);湖南省自然基金项目(编号:2015JJ6076);湖南省中药粉体与创新药物省部共建国家重点实验室培育基地资助

摘　　要：目的研究加味二陈汤对Apo E-/-小鼠血清、主动脉壁及瓣膜的IL-6、TNF-α表达的影响。方法40只Apo E-/-小鼠中10只为空白对照组,30只以高脂饲喂养4周构建动脉粥样硬化(As)小鼠模型,均分成模型组、辛伐他汀组、加味二陈汤组,分别用生理盐水、辛伐他汀及加味二陈汤对小鼠灌胃给药,8周后处死小鼠,Elisa法检测血清中IL-6、TNF-α的含量;免疫组化法检测IL-6、TNF-α在主动脉壁及瓣膜的表达。结果辛伐他汀组血清IL-6和TNF-α分别为392.06±37.70 pg/m L、189.1±50.4 pg/m L,与模型组相比表达下降(P<0.05);加味二陈汤组血清IL-6、TNF-α分别为442.41±172.26 pg/m L、47.2±13.3 pg/m L,与模型组相比表达下降(P<0.05),与辛伐他汀组相比,表达下降(P<0.05);免疫组化结果显示加味二陈汤可降低IL-6、TNF-α在主动脉壁内的表达。结论加味二陈汤可降低血清和主动脉壁及瓣膜的IL-6、TNF-α的表达。Objective This study was to investigate the effects of Modified Erchen Decoction on the expression of IL-6,TNF-αin the aortic wall and serum of ApoE-/ - mice,in order to confirm the effect on the regulation of inflammatory factors in atherosclerosis. Methods ApoE-/ - mice of the control group （n=10） were fed with normal diet,the others （n=30） were fed with a high-cholesterol diet in 4 weeks to induce atherosclerosis mode,and then were divided into the model group （n=10）,the Modified Erchen Decoction group （n=10） and the simvastatin group （n=10）. The two treatment groups were given Modified Erchen Decoction and simvastatin by gavage respectively. The expression of IL-6 and TNF-αwas measured by ELISA kit after treated with the drugs for 8 weeks continuously. Results IL-6 and TNF-α in serum were 392. 06 ± 37. 70 pg/mL and 189. 1 ± 50. 4 pg/mL in the simvastatin group and were 47. 2 ± 13. 3 pg/mL and 442. 41 ± 172. 26 pg/mL in the Modified Erchen Decoction group respectively. They were decreased by the two drugs,and significant difference was found between each treatment group and the control group. The expression of IL-6 and TNF-α were decreased by the two drugs,which were confirmed by the histopathology. Conclusion Modified Erchen Decoction can inhibit the expression of IL-6 and TNF-αeffectively in the ApoE-/ - mice,which indicates that Chinese formula may reduce atherosclerosis progress.

关 键 词：加味二陈汤 动脉粥样硬化 TNF-Α IL-6 

分 类 号：R541.4[医药卫生—心血管疾病]