损伤性肾匀浆体外诱导大鼠骨髓间充质干细胞转分化效果及可能机制的研究  

Transdifferentiation of rat bone marrow mesenchymal stem cell induced by injured kidney homogenate in vitro and the mechanism study

作　　者：陈昌金[1] 杨雪梅[2] 吕春燕[3] 赵梓亦[1] 袁惠[1] 辜海英[1] 高泓[1] 陈奕名[4] 陈明[2] 

机构地区：[1]成都中医药大学附属医院中心实验室,成都610072 [2]成都中医药大学附属医院肾病内科,成都610072 [3]四川省成都市第五人民医院病理科,611130 [4]四川省成都市树德中学光华校区,610091

出　　处：《重庆医学》2016年第3期299-302,共4页Chongqing medicine

基　　金：成都市科技局项目(313-106);成都中医药大学附属医院项目(Y2009003)

摘　　要：目的利用损伤性肾匀浆体外模拟急性肾损伤的微环境,探讨骨髓间充质干细胞(BMSC)诱导转分化及其可能机制。方法将体外培养的BMSC用10%大鼠缺血再灌注损伤肾匀浆上清液进行培养。7d后从形态学改变、18型角蛋白(CK-18)、肝细胞生长因子(HGF)、骨形态发生蛋白-7(BMP-7)的相应表达水平。结果损伤肾匀浆诱导7d后,骨髓间充质干细胞形态发生明显变化,并检测到CK-18阳性表达;同时被诱导的HGF和BMP-7表达水平明显升高。结论损伤肾匀浆可诱导BMSC部分向肾小管上皮样细胞转分化,其可能机制是BMSC可能以旁分泌的形式产生了HGF和BMP-7等"肾脏保护因子"。Objective To explore the effect of transdifferentiation of bone marrow- derived mesenchymal stem cells（BMSCs） and the possible mechanism,the micro-environment of acute kidney injury in vitro was simulated with injured kidney homogenate. Methods BMSCs,isolated from rat bone marrow,were cultured with the presence of 10% injured kidney homogenates. After seven days culturing,the morphological changes were observed under microscope. The expression of CK-18, hepatocyte growth factor （HGF） and bone morphogenetic proteins-7（BMP-7） were tested by qPCR and Western blot respectively. Results The morphologi- cal observation showed that the BMSCs of induced group had been changed obviously after 7 days incubation. The expression of CK- 18 induced group were significantly upregulated. The protein levels of HGF and BMP-7 both in cell culture medium and in induced cell were increased sharply. Conclusion BMSCs could be transdifferentiated partly into renal tubular-like epithelial cells after incu- bation. It figures out the possible mechanism that BMSCs may produce ＂kidney protection factors such as HGF and BMP-7 by their paracrine.

关 键 词：间质干细胞 上皮细胞 肾小管 骨髓间充质干细胞 急性肾损伤 转分化 

分 类 号：R692[医药卫生—泌尿科学]