谷胱甘肽对微囊藻毒素-LR所致小鼠肾脏氧化损伤的保护作用研究  

The protective effects of glutathione on renal oxidative damage induced by microcystin-LR on mice

作　　者：韩知峡[1] 何梅燕 崔琰[1] 熊伟[1] 张青碧[1] 

机构地区：[1]四川医科大学环境卫生与劳动卫生学教研室,四川泸州646000 [2]四川医科大学公共卫生学院预防医学系,四川泸州646000

出　　处：《重庆医学》2016年第3期320-322,共3页Chongqing medicine

基　　金：四川省卫生厅自然科学基金项目(120384);泸州医学院大学生科研项目(2012075)

摘　　要：目的研究谷胱甘肽(GSH)对微囊藻毒素-LR(MC-LR)致小鼠肾脏氧化损伤的保护作用。方法用随机数字表法将40只健康清洁级昆明小鼠分为5组,分别为生理盐水组、GSH对照组、MC-LR染毒组、GSH低剂量+MC-LR染毒组、GSH高剂量+MC-LR染毒组,每组8只(雌雄各半),经腹腔注射染毒,每日1次,持续15d,取出肾脏用于病理观察及检测过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和谷胱甘肽(GSH)、丙二醛(MDA)含量。结果与生理盐水组比较,MC-LR染毒能引起MDA含量[(2.31±0.22)nmol/mg prot]明显升高(P=0.000),以及GSH含量[(0.68±0.02)mg/g prot]、CAT活力[(320.54±38.99)nmol/mg prot]、SOD活力[(180.93±15.30)U/mg prot]、GSH-Px活力[(295.11±42.40)U/mg prot]下降(P<0.05);而外源性加入GSH干预后,与MC-LR染毒组比较,GSH高剂量+MC-LR染毒组MDA含量[(1.94±0.12)nmol/mg prot]明显下降(P<0.05),GSH低、高剂量+MC-LR染毒组GSH含量[(1.01±0.08)mg/g prot、(1.08±0.16)mg/g prot]、CAT活力[(383.46±21.98)nmol/mg prot、(428.50±28.61)nmol/mg prot]均明显升高(P<0.05),GSH高剂量+MC-LR染毒组SOD活力[(222.01±11.51)U/mg prot]、GSH-Px活力[(358.37±20.29)U/mg prot]活力均明显升高(P<0.05)。结论 MC-LR可能通过促进肾脏细胞发生脂质过氧化反应而引起导致肾脏氧化损伤,而加入GSH则可能通过减少脂质过氧化物质,提高抗氧化物活力,清除氧自由基而达到一定的肾脏保护作用。Objective To study the protection of glutathione （GSH） on renal oxidative damage to mice which caused by mi- crocystin-LR（MC-LR）. Methods Forty healthy KM mice were divided into five groups by randomly sampling, which were saline control group,GSH control group,MC-LR group,low dose GSH 4-MC-LR group and high dose GSH 4-MC-LR group,and the ex- periment was lasting 15 days by intraperitoneal injection. Then we took out the kidney for pathological observation and detected the activity of CAT,SOD,GSH-Px and the content of GSH, MDA. Results Compared with control group, the MC-LR increased the content of MDA[（2.31±0.22）nmol/mg prot,P=0. 000] and decreased the content of GSH[（0.68±0.02）mg/g prot]. The activi- ty of CAT[（320.54±38.99）nmol/mg prot], SOD[（ 180.93 ± 15.30） U/mg prot], GSH-Px[（295.11 ± 42.40） U/mg prot] （P〈 0.05）. However,after GSH was given, compared with MC-LR group, MDA content[（1. 94 ±0. 12）nmol/mg prot] of high dose GSH＋MC-LR group significantly decreased （P〈0.05）, GSH content[（1.01 ±0.08）mg/g prot, （1. 08±0. 16）mg/g prot]and CAT activity[ （383.46 ± 21.98） nmol/mg prot, （428.50± 28.61） nmol/mg prot] of both GSH groups significantly increased （ P〈 0.05） ,the activity of SOD[（222. 01±11.51）U/mg prot] and GSH-Px[（358. 37±20.29）U/rag prot] of high dose GSH ＋MC-LR group significantly increased （P〈0.05）. Conclusion MC-LR may cause renal oxidative damage through promoting the lipid perox- idation on renal cells. The GSH may reach a certain protective effect on kidney by reducing the lipid peroxidation,improving the an- tioxidant activity,and removing oxygen free radicals.

关 键 词：肾 谷胱甘肽 微囊藻毒素-LR 氧化损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]