大鼠肝缺血再灌注后肺细胞凋亡及七氟醚对细胞凋亡及NF-κBp65的影响  

Pneumocyte apoptosis after hepatic ischemia- reperfusion in rats and the effect of sevoflurane on apoptosis and NF- κBp65

作　　者：管小萌 徐桂萍[2] 王晓丽[1] 

机构地区：[1]新疆医科大学研究生学院 [2]新疆维吾尔自治区人民医院麻醉科,新疆乌鲁木齐830000

出　　处：《安徽医药》2015年第12期2272-2275,共4页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金(No 81160016)

摘　　要：目的探讨七氟醚预处理对大鼠肝缺血再灌注后肺细胞凋亡的影响。方法 24只Wister大鼠随机分为3组(n=8):假手术组,缺血再灌注组,七氟醚组,阻断肝门30 min后建立大鼠70%肝缺血再灌注模型。假手术组(S组)仅游离肝门,但不阻断;肝缺血再灌注组(IR组)采用阻断肝门30 min,再灌注1 h的方法制备大鼠肝缺血再灌注损伤模型;七氟醚预处理组(SP组)吸入2.1%七氟醚30 min,停止吸入10 min后制备肝缺血再灌注模型。于再灌注1 h时处死动物,留取肺组织,测定湿/干重比(W/D比),采用比色法检测超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,采用原位末端转移酶法(TUNEL)检测细胞凋亡,计算细胞凋亡指数(AI),采用Western blot法测定核蛋白NF-κBp65表达,光镜下观察肺组织病理学结果。结果与S组比较,IR组和SP组再灌注各时点肺组织W/D比值、细胞凋亡指数(AI)和NF-κB活性水平及MDA含量均显著增高(均P<0.05);SOD活性显著降低(P<0.05);与IR组比较,SP组W/D比值、细胞凋亡指数(AI)、NF-κB表达水平与MDA含量显著降低(均P<0.05);而SOD活性显著增加(P<0.05);SP组肺组织损伤较IR组减轻。结论细胞凋亡可能在肝缺血再灌注肺损伤发生过程中具有重要意义;七氟醚对大鼠肝缺血再灌注后肺细胞凋亡具有抑制作用,其作用机制可能通过降低肺组织NF-κB的活性,从而清除自由基、抑制脂质过氧化有关。Objective To explore the influences of sevoflurane pretreatment on apoptosis of rat lung cells after hepatic ischemia reperfusion.Methods Twenty-four Wister rats were randomized into 3 groups（ n = 8） ： sham- operation group,hepatic ischemia reperfusion group and sevoflurane group. After 30 min of hepatic portal occlusion,rat models with 70% hepatic ischemia and reperfusion were established. Porta hepatis was only separated but not blocked in the sham- operation group（ group S）; hepatic ischemia reperfusion rat model was established by 30 min of hepatic portal occlusion and 1 h of reperfusion in hepatic ischemia reperfusion group（ group IR）; In sevoflurane pretreatment group（ group SP）,the rats were inhaled with 2. 1% of sevoflurane for 30 min,and then stopped. 10 min later,hepatic ischemia reperfusion model was established.The animals were executed at 1h after reperfusion. The lung tissue was taken. Wet / dry weight ratio（ W/ D ratio） was determined. Colorimetry was adopted to determine activity of superoxide dismutase（ SOD） and content of malondialdehyde（ MDA）. Terminal- deoxynucleoitidyl transferase mediated nick end labeling（ TUNEL） was used to determine cell apoptosis. Cell apoptosis index（ AI） was calculated. Western blot was used to determine expressions of NF-κBp65. Pathological results of lung tissue were observed under microscope. Results W/ D ratios,AIs,NF-κB activity and MDA content of group IR and group SP at each time after reperfusion were significantly increased compared with group S（ All P 0.05）; SOD activity was decreased significantly（ P〈0. 05）; W/ D ratios,AIs,NF-κB activity and MDA content of group SP were significantly lower than those of group IR（ All P〈0. 05）; and SOD activity was obviously increased（ P〈0. 05）; lung tissue injury of group SP was decreased compared with group IR. Conclusions Cell apoptosis may play an important role in lung tissue injury due to hepatic ischemia reperfusion.Sevoflurane has a certain inhibition on

关 键 词：七氟醚 细胞凋亡 缺血再灌注 

分 类 号：R614[医药卫生—麻醉学]