HPLC-DAD法测定“武当三号金银花”不同部位中芦丁和木犀草苷的含量  

Determination of the contents of rutin and luteoloside in different parts of “Wudang Ⅲ Flos lonicerae”by HPLC- DAD

作　　者：郑芳[1] 朱雪松[1] 李鹏[1] 李志浩[1] 李春雷[1] 李聪[1] 

机构地区：[1]湖北医药学院附属东风医院药学部武当特色中药研究湖北省重点实验室,湖北十堰442008

出　　处：《安徽医药》2015年第12期2302-2306,共5页Anhui Medical and Pharmaceutical Journal

基　　金：湖北省2011协同创新中心基金(鄂教科函(2012)56号)

摘　　要：目的建立HPLC-DAD法测定"武当三号金银花"不同部位中芦丁和木犀草苷的含量。方法采用Fortis Xi Phenyl柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)—0.5%冰醋酸溶液(B)进行线性梯度洗脱;检测波长为354 nm;柱温:30℃。结果芦丁和木犀草苷在各自测定的范围内均呈良好的线性关系(r≥0.999 4),平均回收率分别为99.4%、99.3%。结论该法操作简单,灵敏度高,重现性好,为控制"武当三号金银花"不同部位芦丁和木犀草苷的质量提供了一种可靠的方法。Objective To determine the contents of rutin and luteoloside in different parts of＂Wudang Ⅲ Flos lonicerae＂by HPLC-DAD. Methods Fortis Xi Phenyl column（ 250 mm × 4. 6 mm,5 μm） was used. The mobile phase was acetonitrile（ A）- 0. 5% glacial acetic acid aqueous solution（ B） with gradient elution. The detective wavelength was 354 nm. The column temperature was 30℃. Results The rutin and luteoloside showed good linearity（ r ≥0. 999 4） in a detection range and the average recovery rates were 99. 4%,99. 3%,respectively. Conclusion The method is simple,sensitive and reproducible,which can be used to control the quality of rutin and luteoloside in different parts of ＂Wudang Ⅲ Flos lonicerae＂.

关 键 词：高效液相色谱法 芦丁 木犀草苷 梯度洗脱 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]