白及化学成分与抗肿瘤活性研究  

Chemical Constituents from Bletilla striata and Their Anti-Tumor Activities

作　　者：孙爱静[1,2] 庞素秋[1,2] 王国权[1] 

机构地区：[1]华侨大学生物医学学院分子药物教育部工程研究中心,福建泉州362021 [2]中国人民解放军第180医院临床药学科,福建泉州362000

出　　处：《中国药学杂志》2016年第2期101-104,共4页Chinese Pharmaceutical Journal

摘　　要：目的 研究中药白及的化学成分及其抗肿瘤活性,并对其抗肿瘤活性的作用机制做细胞周期的探讨。方法 采用正、反相硅胶柱、凝胶柱,通过波谱分析进行结构鉴定;采用MTT方法进行化合物的抗肿瘤活性筛选,采用流式细胞仪检测化合物对细胞周期的影响。结果 从中药白及中分离得到5个化合物,经波谱数据分析分别鉴定为齐墩果酸-3-O-α-L-鼠李糖-（1→2）-β-D-吡喃葡萄糖苷（1）,2-羟基丁二酸（2）,对羟基苄胺（3）,棕榈酸（4）和对羟基苯甲酸（5）。化合物1具抗肿瘤活性,且其能够诱导A549细胞周期阻滞于G0/G1期。结论 化合物1-4均为首次从白及中分离得到,其中化合物1具有一定的抗肿瘤活性,其作用机制与细胞周期阻滞相关。OBJECTIVE To investigate the chemical constituents from Bletilla striata （Thunb.） Reichb.f and study their anti-tumor activities and effect on progression of cell cycle. METHODS The compounds were isolated by various chromatographic methods including silica gel, ODS, Sephadex LH-20, and so on. Their structures were identified by extensive analysis of spectiroscopic data. The antiproliferative effects of the compounds were evaluated using MTT test, and compound 1 was tested for the effect on the cell cycle of A549 cells. RESULTS Five compounds were isolated from Bletilla striata and identified as 3β-hydroxyoleane-12-en-28-oic acid 3-O-α-L-rhamnopyranosyl-（1→2）-β-D-glucopyranoside（1）,2-hydroxysuccinic acid（2）,4-hydroxybenzylamine（3）,palmitic acid（4）,and 4-hydroxybenzoic acid（5）. Compound 1 showed antiproliferative activity against the cancer cells and could induce G0/G1 phase arrest effectively after 24 h treatment. CONCLUSION Compounds 1-4 are isolated from Bletilla striata for the first time. Compound 1 shows potent inhibitory effect and might produce their action through inducing cell cycle arrest.

关 键 词：白及 齐墩果酸-3-O-α-L-鼠李糖-(1→2)-β-D-吡喃葡萄糖苷 2-羟基丁二酸 对羟基苄胺 抗肿瘤活性 细胞周期阻滞 

分 类 号：R284[医药卫生—中药学]