玉麦microRNAs类活性物质的筛选及功能分析  

Identification of Bioactivity MicroRNAs Derived from Zea Mays and Their Regulating Target Function

作　　者：王丹仙 马月[2] 方杰[1,2] 胡毅翔 俞文英[2] 余陈欢[2] 应华忠[2] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江省医学科学院,浙江省实验动物与安全性研究重点实验室,杭州310013

出　　处：《中国现代应用药学》2016年第1期12-15,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81473339);浙江省卫生高层次人才培养项目

摘　　要：目的鉴定玉麦中可吸收入血的micro RNAs(mi RNAs)类活性物质,并分析其靶基因功能。方法采用二代高通量测序法,以正常小鼠血浆mi RNAs为空白对照,测定喂食玉麦匀浆液小鼠血浆中mi RNAs的总量,并与玉麦mi RNAs比对,鉴定吸收入体内的玉麦mi RNAs。采用Target Scan与Mi Randa软件,预测玉麦mi RNAs可能作用于人类m RNA靶基因,并通过Gene Ontology(GO)与Kyoto Encyclopedia of Genes and Genomes(KEGG)分析靶基因的相关功能和信号途径。结果共鉴定出12个可吸收入体内的玉麦mi RNAs。GO与KEGG分析显示这些mi RNAs可能调控的靶基因及其参与的生物学功能包括胰岛素信号通路、癌症通路、MAPK通路等相关信号通路,其中mi R4995、mi R156a、mi R396e为玉麦可能的主要核酸活性物质。结论 mi RNAs可能为玉麦中的一种新的活性物质。OBJECTIVE To investigate micro RNAs（miRNAs） derived from Zea mays and their target gene function. METHODS Mi RNA profiles of Zea mays, in plasma of normal mice and Zea mays extract-treated mice were detected by using next generation sequencing, and then the mi RNAs which were took into the organism were validated with mi RNA database of Zea mays. Human m RNA database as targets was used to predicte target genes of mi RNAs by Target Scan and Mi Randa analysis, while the target gene function was considered by Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis. RESULTS Twelve mi RNAs were identified. For the KEGG pathway, their target gene mainly involved in insulin signaling pathway, MAPK pathway and peritumoral pathways by GO and KEGG analysis. The results of mi RNAs-gene-network showed that mi R4995, mi R156 a, mi R396 e might be the main pharmacodynamic substance. CONCLUSION Mi RNAs might act as a bioactivity substance in the Zea mays.

关 键 词：药效物质 高通量测序 靶基因 

分 类 号：R931.6[医药卫生—生药学]