碳青霉烯类抗菌药物对肠杆菌科细菌及非发酵菌的防突变浓度研究  

Mutant prevention concentrations of carbapenem antibiotics against Enterobacteriaceae and nonfermenters

作　　者：李建华[1] 王玉明[2] 戴路明[1] 张力燕[1] 罗壮[1] 孙士波[1] 汪矗[1] 何慧琳[1] 

机构地区：[1]昆明医科大学第一附属医院呼吸内科,云南昆明650032 [2]昆明医科大学第一附属医院检验科,云南昆明650032

出　　处：《中国新药与临床杂志》2016年第1期53-58,共6页Chinese Journal of New Drugs and Clinical Remedies

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金(2013FB145)

摘　　要：目的评价碳青霉烯类抗菌药物对临床分离的100株大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌和鲍曼不动杆菌的防突变浓度(MPC)。方法采用琼脂稀释法测定碳青霉烯类抗菌药物(亚胺培南、美洛培南、厄他培南和多利培南)对临床分离的肠杆菌科和非发酵菌菌株的最小抑菌浓度(MIC)及MPC,并计算MPC/MIC比值。结果碳青霉烯类抗菌药物对肠杆菌科敏感株的MPC为0.25~4.0μg·m L^(-1),耐药株MPC为8.0~>64.0μg·m L^(-1)。多利培南、亚胺培南、美洛培南对非发酵菌敏感株的MPC为2.0~4.0μg·m L^(-1),耐药株MPC为8.0~128μg·m L^(-1)。肠杆菌科细菌和非发酵菌敏感株的MPC/MIC比值为2~4,耐药株多为8~>16。结论碳青霉烯类抗菌药物对肠杆菌科和非发酵菌敏感株的MPC为0.125~4.0μg·m L^(-1),MPC/MIC比值为2~4;对耐药株的MPC为2.0~128.0μg·m L^(-1),MPC/MIC比值为8~>16。AIM To evaluate the mutant prevention concentration （MPC） of carbapenem antibiotics against 100 strains of isolated clinically Escherichia coli, KlebsieUa pneumoniae , Pseudomonas aeruginosa and Bauman Acinetobacter. METHEODS Agar dilution method was used to determine the minimal inhibitory concentration （MIC） and MPC of carbapenems （imipenem, meropenem, ertapenem and doripenem） against isolated clinically Enterobacteriaceae and non-fermentative bacteria strains, and calculate the ratio of MPC/MIC. RESULTS MPC of carbapenem antibiotics was 0.25 - 4.0 μg·mL^-1 for sensitive strains of Enterobacteriaceae and 8.0 -〉 64.0 μg·mL^-1 for resistant strains. MPC of doripenem, imipenem, and meropenem for sensitive strains of non-fermentative bacteria was 2.0 - 4.0 μg·mL^-1, and 8.0 - 128μg·mL^-1 for resistant strains. MPC/ MIC ratio of Enterobacteriaceae and non-fermentative bacteria was 2 - 4 in sensitive strains and 8 -〉 16 in resistant strains. CONCLUSION MPC of carbapenem antibiotics for sensitive strains of Enterobacteriaceae and nonfermentative bacteria is 0.125 - 4.0 μg·mL^-1 with resistant strains with MPC/MIC ratio 8 -〉 16. MPC/MIC ratio 2 - 4, and 2.0 - 128.0 μg·mL^-1 for

关 键 词：碳青霉烯类 防突变浓度 最小抑菌浓度 Β-内酰胺酶类 肠杆菌科 非发酵菌 微生物敏感性试验 

分 类 号：R978.1[医药卫生—药品]