糖尿病大鼠的骨膜反应及染料木素的保护作用  

Changes of the periosteal reaction of diabetic rat and the protection of genistein

作　　者：陈海英[1] 林冬梅[2] 王希[3] 张趁华[1] 

机构地区：[1]福建莆田学院医学院基础医学部,福建莆田351100 [2]福建莆田学院药学与医学技术学院,福建莆田351100 [3]福建莆田学院临床医学院,福建莆田351100

出　　处：《中国临床解剖学杂志》2016年第1期73-77,共5页Chinese Journal of Clinical Anatomy

基　　金：福建教育厅科研资助项目(JA08189)

摘　　要：目的探讨糖尿病大鼠骨膜反应的动态演变及染料木素(Gen)的保护作用。方法建立速发型链脲佐菌素(streptozotocin,STZ)糖尿病大鼠模型,随机分为正常组(CON);糖尿病组(DM);Gen低剂量干预组(Gen Low);Gen高剂量干预组(Gen High)(30 mg·kg-1·d-1)。每天1次,灌胃给药(4.0 mg/kg)10周,每组20只。对各组大鼠骨膜作组织病理学分析及组织计量学测定;利用Van Gieson胶原纤维染色法观察胶原纤维沉积变化;墨汁灌注行边缘微血管密度测定。结果 DM组骨膜厚度等均明显小于CON组(P<0.01);微血管密度增大,但渗透性大。Gen High骨膜厚度明显减少(P<0.01)。结论糖尿病先后出现骨膜水肿、增生、退化等骨膜反应。Gen对糖尿病的骨膜反应具有改善保护作用。Objective To study the changes of the periosteal reaction of the diabetic rat and the protection of genistein. Methods The diabetic model （STZ） of rat was established and randomly divided into a normal group and adiabetic group, with 10 rats in each group; GenLow group; GenHigh group. 20 rats each group. The change of collagen fiber depositing was observed by Van Gieson collagen fiber staining method and the microvascular density was measured by ink infusion method. Results The thickness of periosteum wasreduced in the 10-week diabetic group than those in the control group（P〈0.01）, the microvascular density and permeability were enlarged. Thickness of periosteum was reduced by GenLow and by GenHigh after 10 weeks of treatment （P〈0.01）. Conclusions With periosteal reaction include periosteal edema, proliferation and regression in the diabetic. Gen could protect the periosteum in the diabetes rats.

关 键 词：糖尿病 骨膜 骨膜反应 染料木素 骨祖细胞 胶原纤维 微血管 墨汁灌注 

分 类 号：R587.2[医药卫生—内分泌]