水貂肺炎混合感染病原菌的分离鉴定  

Isolation and identification of the pathogens causing mixed infections in minks with pneumonia

作　　者：孟洋[1] 齐凯歌[1] 王丽萍[1] 张彦龙[1] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040

出　　处：《黑龙江畜牧兽医》2016年第2期143-145,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家林业局疫源疫病病原体分析与保持项目

摘　　要：为了确定山东省临沂市某水貂养殖场水貂肺炎的病原体,试验从发病死亡水貂体内分离到2株细菌LY8301和LY8302,根据培养特征、生化试验、16S rRNA序列测定及药敏试验对2株细菌进行分析。结果表明:LY8301菌株为绿脓杆菌,LY8302菌株为鲍曼不动杆菌;2株细菌均对头孢噻吩、氨苄西林、青霉素G及四环素耐药,对阿米卡星、环丙沙星、庆大霉素和氧氟沙星较为敏感;2株细菌均对小鼠有较强的致病性,可以确定此次水貂肺炎由鲍曼不动杆菌与绿脓杆菌混合感染引起。To make sure the pathogen of mink with pneumonia at a farm in Linyi of Shandong province, two bacterial strains including LY8301 and LY8302 were isolated from the body of dead diseased mink. The strains were analyzed by culture characteristics, biochemical test, se- quence determination of 16S rRNA, and drug sensitivity test. The results showed that LY8301 was identified as Pseudorrmnas aeruginosa, and LY8302 was identified as Acinetobacter baumannii. Both strains were resistant to eefalotin, ampiciUin, penicillin G and tetracycline, and more sensitive to amikacin, ciprofloxacin, gentamycin and ofloxacin. The two strains had strong pathogenicity to mice. It was confirmed that pneumonia in minks was caused by the mixed infection of Pseudomonas aeruginosa and Acinetobacter baumannii.

关 键 词：水貂 绿脓杆菌 鲍曼不动杆菌 混合感染 分离鉴定 肺炎病原体 

分 类 号：S865.22[农业科学—野生动物驯养] S852.61[农业科学—畜牧兽医]