人类白细胞抗原DQ基因多态性与不明原因复发性流产的关联分析  被引量:20

Analysis of the association of human leukocyte antigen DQ gene polymorphisms with unexplained recurrent spontaneous abortion among ethnic Han Chinese from Wenzhou region

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作  者:郑加永 张红萍 徐晓敏 马卫德 李建新 夏淑琦 汪海 沈晓露 

机构地区:[1]浙江省温州市人民医院、温州市妇幼保健院妇产科实验室,温州市妇产科学重点实验室,325000

出  处:《中华医学遗传学杂志》2016年第1期81-84,共4页Chinese Journal of Medical Genetics

基  金:浙江省自然科学基金(Y13H040023);浙江省医药卫生科技计划平台重点资助项目(2013ZDA023);温州市科技计划项目(Y20140040);温州市医药卫生科学研究项目(2013806)

摘  要:目的探讨温州地区汉族夫妇人类白细胞抗原(humanleukocyteantigen,HLA)DQ基因多态性与不明原因复发性流产(unexplainedrecurrentspontaneousabortion,URSA)的关系。方法应用聚合酶链反应序列特异引物法分析50对URSA夫妇(URSA组)和66对正常妊娠史夫妇(对照组)HLA-DQAl及DQBl等位基因,分析各等位基因分布频率、比值比(oddsratio,OR)及夫妇双方HLA_DQAl及DQBl等位基因相容性。结果URSA组女性HLA-DQBl*03:03等位基因频率为21.00%,与对照组女性的9.85%比较,差异有统计学意义(OR=2.433,95%C1:1.232~4.894,X2=5.657,P〈0.05)。对照组女性HLA-DQBl*05:03等位基因频率为3.03%,在URSA组女性中未检出。对照组男性、女性HLA—DQBl*05:02等位基因频率分别为6.06%和5.30%,而URSA组夫妇中均未发现。URsA组夫妇共有HLA—DQAl等位基因占70.27%,高于对照组夫妇(44.64%),差异有统计学意义(OR=2.931,95%CI:1.216~7.067,X2=5.908,P〈0.05)。结论温州地区汉族人群中URSA可能存在遗传易感性,且与夫妇双方HLA-DQAl等位基因相容性增高有关;女性HLA—DQBl*03:03等位基因可能是URSA易感等位基因,夫妇双方HLA—DQBl*05:02等位基因及女性HLA-DQBl*05:03等位基因可能是URSA保护基因。Objective To assess the association of human leukocyte antigen DQ gene polymorphisms with unexplained recurrent spontaneous abortion (URSA) among ethnic Han Chinese from Wenzhou region. Methods Fifty couples with URSA (URSA group) and 66 couples with normal pregnancy history (control group) were recruited. The alleles of HLA-DQA1 and HLA-DQB1 were analyzed by polymerase chain reaction with specific sequence primers (PCR-SSP) in all subjects. The frequency distribution of HLA-DQ alleles, odds ratios (OR) between each group and sharing of HLA-DQ alleles were calculated. Results The frequency distribution of HLA-DQB1 * 03:03 allele in the females with URSA was significantly higher than that healthy females (21.00% vs. 9.85%, 0R:2.433, 95%CI.. 1. 232-4. 894, X2=5.657, P〈:0.05). The HLA-DQB1 * 05:03 allele was present among the healthy females with a frequency of 3.03%, and was not detected among females with URSA. For both males and females, the HLA-DQB1 * 05:02 allele were only typed in control group with frequencies of 6. 06% and 5. 30%, respectively. The sharing of HLA- DQA1 alleles in couples with URSA was increased compared with the control group (70. 27% vs. 44.64%, OR=2. 931, 95~CI: 1. 216-7. 067, P〈0.05). Conclusion The increased sharing of HLA-DQA1 alleles may contribute to the susceptibility of URSA among ethnic Han Chinese from Wenzhou region. The allele of HLA-DQB1 * 03:03 in the females may be predisposing factor for URSA. However, the HLA-DQB1 * 05: 02 allele in both gender and HLA-DQB1 * 05:03 allele in females may confer a protective effect.

关 键 词:复发性流产 人类白细胞抗原 HLA-DQA1基因 HLA-DQB1基因 

分 类 号:R714.21[医药卫生—妇产科学]

 

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