吉西他滨干预荧光素钾的乳腺癌细胞胞内外排动力学研究  

Effect of gemcitabine on efflux kinetics of luciferin potassium in breast cancer cells

作　　者：孙悦[1] 谷满仓[1] 钱亚芳[2] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江省中医院制剂中心,杭州310006

出　　处：《中国新药杂志》2016年第2期198-201,209,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金青年基金项目(81303235);教育部高等学校博士学科点专项科研基金项目(20113322120001);浙江省自然科学基金青年基金项目(LQ12H29002);浙江省医药卫生科技项目(2011KYA119);教育部国家大学生创新创业训练计划(201310344002)

摘　　要：目的:评价吉西他滨对荧光素钾在携带荧光素酶报告基因的乳腺癌细胞株4T1luc胞内的外排动力学的影响。方法:以4T1luc细胞为模型,经不同浓度吉西他滨干预24 h后加入50μg·m L﹣1荧光素钾,应用生物发光成像技术连续动态检测各处理组生物发光信号强度随时间变化过程,计算荧光素钾的外排动力学参数。结果:荧光素钾浓度与生物发光信号光子量呈正相关。随着吉西他滨处理浓度增加,K逐渐增加,AUC逐渐降低(P<0.05)。结论:吉西他滨能够促进乳腺癌细胞胞内荧光素钾的外排动力学过程。Objective： To evaluate the intervention of gemcitabine（ GEM） on efflux kinetics of luciferin potassium in luciferase reporter gene over-expressed 4T1 breast cancer cells（ 4T1luc）. Methods： Using 4T1 luccells as a model,50 μg·m L- 1luciferin potassium solution was added after 24 h intervention by different concentrations of gemcitabine. Then the bioluminescent signal intensity was detected continuously and dynamically in each treatment group with bioluminescence imaging technology,and the efflux kinetic parameters of luciferin potassium were calculated. Results： The concentration of luciferin potassium was positively related to bioluminescent signal photon.With the increase of gemcitabine concentration,K increased and AUC decreased compared to the control group（ P 〈0. 05）. Conclusion： Gemcitabine can promote the efflux of luciferin potassium in breast cancer cells.

关 键 词：吉西他滨 荧光素钾 乳腺癌细胞 生物发光成像 药动学 

分 类 号：R979[医药卫生—药品] R737.9[医药卫生—药学]