茄青枯拉尔氏菌Rs-T02在3,4,5-三羟基苯甲酸甲酯作用下的差异蛋白分析  

Differential protein analysis of Ralstonia solanacearum strain Rs-T02 after methyl gallate treatment

作　　者：范腕腕[1] 余功明[1] 袁高庆[1] 林纬[1] 魏昌英 黎起秦[1] 

机构地区：[1]广西大学农学院,南宁530004

出　　处：《植物病理学报》2016年第1期47-55,共9页Acta Phytopathologica Sinica

基　　金："国家自然科学基金"(31460459)

摘　　要：为探明3,4,5-三羟基苯甲酸甲酯(MG)抑制茄青枯拉尔氏菌的机制,利用二维电泳技术,分析了MG作用下茄青枯拉尔氏菌Rs-T02菌株的差异蛋白。结果发现在MG作用下的菌体中检测出29个差异蛋白点。采用质谱技术鉴定出22个差异蛋白,其中新增蛋白11种、缺失蛋白5种、表达上调蛋白1种和表达下降蛋白5种。这些蛋白参与能量代谢、信号转导、物质运输和DNA修复等途径。我们推测,与能量代谢相关的新增蛋白spot 9和缺失蛋白spot 65可能与MG抑制茄青枯拉尔氏菌的机制有关。In order to explain the anti-bacterial mechanism of methyl gallate （MG） in Ralstonia solanacearum strain Rs-T02, two-dimensional gel electrophoresis （2-DE） was used to seperate the differential protein of the pathogen with MG treatment. The results showed that 29 differential protein spots were detected. Twenty two proteins, which included 11 newly-emerged, 5 disappeared, 1 up-regulated and 5 down-regulated proteins,were identified by LC-MS/MS. These proteins were related to energy metabolism, signal transduction and transloca- tion of solute or DNA repair. We hypothesized that newly-emerged protein spot 9 and disappeared protein spot 65, which were related to the energy metabolism, might involve in the anti-bacterial mechanism of MG in R. So- lanacearum.

关 键 词：茄青枯拉尔氏菌 3 4 5-三羟基苯甲酸甲酯 差异蛋白 

分 类 号：S432.42[农业科学—植物病理学]