茎枯病菌毒素制备及对芦笋愈伤组织抗性诱导的研究  

Phomopsis asparagi toxin preparation and the resistance induction on asparagus callus by the pathogen toxin

作　　者：李俊萍[1] 乜兰春[1] 王珊珊[1] 刘孟[1] 

机构地区：[1]河北农业大学园艺学院,保定071000

出　　处：《植物病理学报》2016年第1期140-144,共5页Acta Phytopathologica Sinica

基　　金：基金项目:河北省现代农业产业技术体系蔬菜创新团队

摘　　要：Toxin-producing culture medium and culture time for Phomopsis asparagi were optimized, and theeffect of the pathogen toxin on asparagus callus was investigated in the study. It was found that the toxin pro-duced from culture for 12 days in the Fries medium showed the strongest inhibition effect on asparagus radicle.Toxin caused callus of ＇ Apollo＇ , ＇ Champion＇ and ＇ NJ987＇ browning. Resistance of the callus of the threevarieties to toxin could be induced by toxin treatment. Toxin concentration for resistant callus selection was30% -40%.Toxin-producing culture medium and culture time for Phomopsis asparagi were optimized,and the effect of the pathogen toxin on asparagus callus was investigated in the study.It was found that the toxin produced from culture for 12 days in the Fries medium showed the strongest inhibition effect on asparagus radicle.Toxin caused callus of 'Apollo','Champion' and 'NJ987' browning.Resistance of the callus of the three varieties to toxin could be induced by toxin treatment.Toxin concentration for resistant callus selection was 30%-40%.

关 键 词：PHOMOPSIS asparagi toxin RADICLE inhibition rate CALLUS BROWNING 

分 类 号：S[农业科学]