HPLC-荧光法测定大鼠微量血浆中酒石酸唑吡坦的含量  

Content Determination of Zolpidem Tartrate in Microsamples of Rat Plasma by HPLC-fluorescence Method

作　　者：苗彩云[1] 陈江飞[2] 朱素燕[2] 徐萍[2] 

机构地区：[1]宁波市妇女儿童医院药剂科,浙江宁波315012 [2]宁波市第一医院药剂科,浙江宁波315010

出　　处：《中国药房》2016年第4期468-470,共3页China Pharmacy

基　　金：2013年浙江省医学会临床科研基金项目(No.2013ZYC-A68)

摘　　要：目的:建立微量血浆中酒石酸唑吡坦的含量测定方法。方法:取大鼠,ig酒石酸唑吡坦溶液3 mg/kg,给药后取血0.2 ml,分离后取血浆50μl经甲醇沉淀蛋白,取上清液采用高效液相色谱-荧光法测定,以外标法进行定量。色谱柱为Agilent HC-C18,流动相为0.03 mol/L磷酸二氢钾溶液(含0.2%三乙胺)-甲醇(33∶67,V/V),流速为1.0 ml/min,激发波长为254 nm,发射波长为390nm,进样量为20μl。结果:酒石酸唑吡坦检测质量浓度的线性范围为2~200μg/L(r=0.999 7),定量下限为2μg/L;方法回收率为(96.96±1.35)%^(105.0±5.36)%(RSD为2.20%~4.88%,n=5);提取回收率为(79.72±0.01)%^(80.77±0.02)%(RSD为1.34%~3.90%,n=5);日内RSD为1.40%~5.10%,日间RSD为3.22%~9.25%(n=5)。结论:本法简便、灵敏,可用于微量血浆中唑吡坦的含量测定。OBJECTIVE：To establish a method for the content determination of zolpidem tartrate in microsamples of rat plasma. METHODS：Rats were given zolpidem tartrate solution 3 mg/kg intragastrically,and 0.2 ml blood sample were collected and isolated. 50 μl plasma was precipitated by methanol,and the supernatant was determined by HPLC-fluorescence combined with external method. Agilent HC-C18 column was used with mobile phase consisted of 0.03 mol/L KH2PO4solution（containing 0.2% triethylamine）-methanol（33 ∶ 67,V/V） at flow rate of 1.0 ml/min. The excitation and emission wavelengths were 254 nm and 390 nm,respectively. The sample size was 20 μ l. RESULTS：The linear ranges of zolpidem tartrate in plasma was 2-200 μ g/L（r=0.999 7）,and the limit of quantification was 2 μg/L. The method recoveries of zolpidem were（96.96±1.35）%-（105.0±5.36）%（RSD=2.20%-4.88%,n=5）,and extraction recoveries were（79.72±0.01）%-（80.77±0.02）%（RSD=1.34%-3.90%,n=5）.The intra-day and inter-day RSDs were 1.40%-5.10% and 3.22%-9.25%（n=5）,respectively. CONCLUSIONS：The method is simple,sensitive and suitable for the content determination of zolpidem tartrate in microsamples of plasma.

关 键 词：高效液相色谱法 酒石酸唑吡坦 微量血浆 血药浓度 大鼠 

分 类 号：R965[医药卫生—药理学]