正交试验法优化痛风消颗粒水提醇沉工艺  

Optimization of Water Extraction and Alcohol Precipitation Technology of Tongfengxiao Granules by Orthogonal Test

作　　者：李卿[1] 梁旭明[1] 杜洪飞[1] 周兴[1] 王颖[2] 郭剑华[3] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]重庆市中医院药剂科,重庆400021 [3]重庆市中医骨科医院,重庆400010

出　　处：《中国药房》2016年第4期518-521,共4页China Pharmacy

基　　金：重庆市渝中区科技计划项目(No.20120223)

摘　　要：目的:优化痛风消颗粒的水提醇沉工艺。方法:采用正交试验法,以盐酸小檗碱、丹酚酸B含量为指标,考察加水量、提取时间、提取次数对水提取工艺的影响;以盐酸小檗碱、丹酚酸B保留率为指标,考察提取液相对密度、含醇量、醇沉静置时间对醇沉工艺的影响;同时进行验证试验。结果:最优水提取工艺为加8倍量水、煎煮3次、每次60 min;最优醇沉工艺为提取液浓缩至相对密度1.15、冷却后加入乙醇使含醇量为80%、静置48 h,除去沉淀。验证试验结果表明,提取后小檗碱、丹酚酸B含量平均值分别为6.344 6、7.312 2 mg/g(RSD=2.46%、1.08%,n=3),保留率分别为91.03%、92.39%(RSD=1.72%、1.50%,n=3)。结论:优化的水提、醇沉工艺稳定可行、重现性好,适用于本制剂的提取、分离和纯化。OBJECTIVE：To optimize water extraction and alcohol precipitation technology of Tongfengxiao granules. METHODS：The effects of the amount of water,extraction time and extraction times on water extraction technology were investigated by orthogonal test using the content of berberine hydrochloride and salvianolic acid B as index. The effects of the relative density of liquid,alcohol content and processing time on alcohol precipitation technology were investigated by orthogonal test using the retention rate of berberine hydrochloride and salvianolic acid B as index. Validation test was conducted. RESULTS：The optimal water extraction technology was as follows as 8-fold water,decocting for 3 time,lasting for 60 min each time. The optimal alcohol precipitation technology was as follows as relative density of extraction liquid concentrated to 1.15,the content of alcohol 80% after adding alcohol following cooling,placing for 48 h,removing precipitation. Results of validation test indicated the average contents of berberine and salvianolic acid B were 6.344 6（RSD=2.46%,n=3）and 7.312 2 mg/g（RSD=1.08%,n=3）,and retention rate of them were 91.03%（RSD=1.72%,n=3）and 92.39%（RSD=1.50%,n=3）. CONCLUSIONS：The optimized water extraction and alcohol precipitation technology are feasible and producible,and suitable for the extraction,separation and purification of the preparation.

关 键 词：痛风消颗粒 水提取 醇沉 正交试验 盐酸小檗碱 丹酚酸B 

分 类 号：R284.2[医药卫生—中药学]