壳寡糖衍生物纳米银微粒诱导烟株产生TMV抗性  

Study on TMV resistance of tobacco plants induced by chitosan oligosaccharide derivatives silver nanoparticles

作　　者：孙翠红 徐翠莲[2] 赵铭钦 黄元炯[3] 黄海棠 朱金峰 

机构地区：[1]烟草学院国家烟草栽培生理生化研究基地,郑州450002 [2]河南农业大学理学院,郑州450002 [3]河南省烟草公司,郑州450002 [4]河南省漯河市烟草公司,漯河462000

出　　处：《中国烟草学报》2015年第6期59-64,共6页Acta Tabacaria Sinica

基　　金：河南省烟草公司重点科技攻关项目[HYKJ201015]和[HYKJ201307]

摘　　要：为开发高效、低毒且环境友好的新型抗烟草普通花叶病毒制剂,以合成的具有季铵盐结构的新型壳寡糖希夫碱衍生物为还原剂和稳定剂合成了壳寡糖衍生物纳米银微粒(C_4-Ag NPs),并探讨了其对烟草花叶病毒的抑制效应及生理生化机制。结果表明17μg/m L的C_4-Ag NPs在珊西烟上对烟草普通花叶病毒具有良好的预防和治疗效果,防效可达到86.95%;C_4-Ag NPs喷施在感染TMV的普通烟K326上可在一定的时间内提高叶片内防御酶过氧化氢酶(CAT)和苯丙氨酸解氨酶(PAL)的活性,使脯氨酸含量得到提高,丙二醛含量降低。C4-AgNPs were synthesized by using new chitosan Schiff base derivatives with the structure of quaternary ammonium salt as reducing agent and stabilizer to develop new anti-TMV agent which was efficient, low toxic and environmental friendly. Inhibitory effect of s C4-AgNPs on TMV and its biochemical mechanism were also studied. Results showed that 17 gg/mL C4-AgNPs had good prevention and treatment effects on TMV, and it could effectively reduce the number of lesion with prevention rate up to 86.95%. C4-AgNPs could improve activity of defense enzymes catalase （CAT） and phenylalanine ammonialyase （PAL）. Meanwhile, the content of proline was enhanced and that of MDA reduced by tracing the changes of physiological and biochemical indexes in a certain period of time on Nicotiana tabacum var. K326.

关 键 词：纳米银 壳寡糖季铵盐衍生物 烟草普通花叶病毒 抑制 机理 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]