线粒体旁路氧化途径抑制剂水杨肟酸(SHAM)协同下烟草赤星病菌对嘧菌酯的敏感性  

Sensitivity of Alternaria alternata to azoxystrobin at the synergism of alternative oxidative inhibitor salicylhydroxamic acid

作　　者：黄艳飞[1] 陈庆园 王进[3] 薛娟娟[1] 王茂胜 鲁红学[1] 商胜华 张长青[1] 汪汉成 

机构地区：[1]长江大学农学院,湖北荆州434025 [2]贵州省烟草科学研究院烟草行业分子遗传重点实验室,贵阳550081 [3]长江大学生命科学学院,湖北荆州434025

出　　处：《中国烟草学报》2015年第6期65-70,共6页Acta Tabacaria Sinica

基　　金：国家自然科学基金(31360448;31501679);中国烟草总公司贵州省公司科技项目(201305;201336;201436);黔西南州烟草公司(2013-06)

摘　　要：测定了线粒体旁路氧化途径抑制剂水杨肟酸(SHAM)协同下烟草赤星病菌对嘧菌酯的敏感性。结果表明:嘧菌酯抑制病原菌菌丝生长和孢子萌发的EC50值分别为25.8300 mg/L和0.0500 mg/L,在100 mg/L SHAM协同下,EC50值分别降低为15.5200和0.0038 mg/L;SHAM对烟草赤星病菌菌丝的生长和分生孢子的萌发没有影响。研究说明SHAM增强了嘧菌酯对烟草赤星病菌的毒力,线粒体电子传递的旁路氧化途径存在于烟草赤星病菌中,且在孢子萌发中的增效强于菌丝生长阶段。This paper investigates the sensitivity of Altemaria alternata to Azoxystrobin in the synergistic effects of alternative oxidative inhibitor salicylhydroxamic acid （SHAM）. Results showed that azoxystrobin inhibited both mycelial growth and conidial germination of A. alternata, with EC50 values of 25.8300 and 0.0500 mg L-1, respectively; while in the combination of 100 mg L-1 SHAM, it showed EC50 values of 15.5200 and 0.0038 mg L-1, respectively. SHAM presented no effects on mycelial growth or conidial germination of the pathogen. Studies showed that SHAM enhanced the sensitivity of A. alternata to azoxystrobin, and alternative oxidative pathway of electron transport chain existed in pathogen A. alternata., especially in the stage of conidial germination.

关 键 词：烟草赤星病菌 嘧菌酯 水杨肟酸 敏感性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]