比色法测定昆明山海棠片中总二萜内酯的含量  

Determination the Content of Total Diterpene Lactones in Kunming Shanhaitang Tablets by Colorimetry

作　　者：江银蒜 刘岩庭[2] 吴春敏[1] 

机构地区：[1]福建中医药大学,福州350122 [2]福建省药品检验所,福州350001

出　　处：《海峡药学》2015年第12期41-43,共3页Strait Pharmaceutical Journal

摘　　要：目的建立测定昆明山海棠片中总二萜内酯含量的方法。方法采用比色法,在545nm波长处进行总二萜内酯的含量测定。结果比色法测定昆明山海棠片总二萜内酯（以雷公藤甲素计）在8.17μg·m L-1~49.1μg·m L-1范围内线性关系良好（r=0.9999）,平均加样回收率为99.2%,RSD为1.94%（n=6）。结论本法简便、准确,适用于昆明山海棠片中总二萜内酯的含量测定,不同企业的昆明山海棠片中总二萜内酯的含量差异较大,在临床用药时应引起重视。OBJECTIVE To determine the content of total diterpene lactones in Kunming Shanhaitang tablets. METHODS A colorimetric method was performed to determine the content of total diterpene lactones at545 nm. RESULTS The calibration curves were linear in the range from 8. 17μg ·m L- 1to 49. 1μg ·m L- 1（ r =0. 9999） for total diterpene lactones（ calculated as triptolide） with colorimetric method. The average recovery rate（ n = 6） was 99. 2% with RSD as 1. 94%. CONCLUSION The method is simple and accurate,and can be used in the determination of Kunming Shanhaitang tablets. The content of total diterpene lactones in Kunming Shanhaitang tablets from different factories were compraratively largely disparity,which should be taken into account in clinic.

关 键 词：昆明山海棠片 总二萜内酯 比色法 

分 类 号：R927[医药卫生—药学]