HPLC法同时测定独红骨刺丸中三七的3种成分  

Simultaneous Determination of Three Chemical Contents of Notoginseng in Duhong Guci Pills by HPLC

作　　者：马昌豪 李洪芹[2] 

机构地区：[1]山东省菏泽市食品药品检验检测中心,菏泽274000 [2]菏泽学院,菏泽274015

出　　处：《海峡药学》2015年第12期57-59,共3页Strait Pharmaceutical Journal

摘　　要：目的建立同时测定独红骨刺丸中三七3种成分的方法。方法应用高效液相色谱法,色谱条件为Thermo C18色谱柱,乙腈-水为流动相梯度洗脱,体积流量1.0m L·min-1,检测波长203nm。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1线性范围分别为0.04-1μg,r2=0.9957;0.1068-2.67μg,r2=0.9994;0.1034-2.585μg,r2=0.9998。平均加样回收率分别为101.31%（RSD=1.90%）;98.91%（RSD=1.20%）;97.74%（RSD=0.97%）。结论本方法操作简便,重复性好,能有效地控制该制剂的质量。OBJECTIVE To establish a HPLC method for simultaneous determination the contents of notoginseng in Duhongguci Pills. METHODS HPLC was applied with the chromatographic conditions as follows： the chromatographic column was Thermo C18,Acetonitrile-water as the mobile phrase,flow rate was 1. 0m L·min- 1. The detection wavelength was set at 203 nm. RESULTS The linear rang of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 were 0. 04 - 1μg,r2= 0. 9957; 0. 1068 - 2. 67μg,r2= 0. 9994 and 0. 1034 - 2. 585μg,r2= 0. 9998,respectively and their average recoveries were101. 31%（ RSD = 1. 90%）; 98. 91%（ RSD = 1. 20%）; 97. 47%（ RSD =0. 97%）. CONCLUSION The method was simple,reproducible and it was suitable for the quality control of Duhong guci Pills.

关 键 词：独红骨刺丸 三七 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]