广西主栽木薯品种体细胞胚的观察  

Study on Somatic Embryogenesis of Manihot esculenta Cultivars in Guangxi

作　　者：黎桦[1] 黄贤教 林丽红[2] 刘峰[2] 钱梦丹 陈英之[1] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西大学生命科学与技术学院,广西南宁530005

出　　处：《西南农业学报》2015年第6期2408-2412,共5页Southwest China Journal of Agricultural Sciences

基　　金：广西教育厅科技创新项目(914科技创新-C4860097901);广西大学科研启动基金项目(DD 702002);广西高校大学生创新创业计划项目(T3130098206)

摘　　要：以广西主栽木薯品种华南124(SC124)、新选048、辐选01以及非洲老品种TMS60444为试验材料,通过对SC124、新选048、辐选01和TMS60444的初级胚状体(华南124没有初级胚状体)、次级胚状体及TMS60444的胚性愈伤组织进行解剖学研究,采用石蜡切片法显微观察四种材料不同阶段胚状体的细胞微观形态。结果发现新选048、TMS60444和辐选01的初级胚状体细胞特征符合原分生组织特点,拥有非常旺盛的分裂能力,这可为鉴别胚性愈伤组织提供理论依据。Four cassava cultivars viz.,SC124,Xinxuan 048 and Fuxuan 01（ main cultivar grown in Guangxi）,and Africa variety TMS60444 were taken as test materials to observe their embryoid cell micromorphology by anatomy research of primary embryoid,secondary embryoid or embryonic callus in present study. The paraffin section observations results showed that the characteristics of primary somatic cells of Xinxuan048,TMS60444 and Fuxuan 01 were accorded with the promeristem character,the cells had very vigours dividing ability,which would laid the theoretical basis for embryonic callus identification.

关 键 词：初级胚状体 次级胚状体 胚性愈伤组织 石蜡切片法 木薯 

分 类 号：S533[农业科学—作物学]