姬菇258和金凤2-1杂交构建姬菇新菌株  

Hybrid Strains Breeding by Pleurotus cornucopiae No. 258and Pleurotus sp. Jin feng2-1

作　　者：谭伟[1] 周洁[1] 曹雪莲[2] 苗人云[2] 贾定洪[1] 黄忠乾[1] 甘炳成[1] 彭卫红[1] 郑林用[1] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066 [2]四川金地菌类有限责任公司,四川成都610066

出　　处：《西南农业学报》2015年第6期2655-2660,共6页Southwest China Journal of Agricultural Sciences

基　　金：四川省"十二五"农作物及畜禽育种攻关品种选育项目子专题"姬菇新品种选育"(2011NZ0098-7);国家现代农业产业技术体系四川食用菌创新团队新品种引育研究岗位建设项目(川农业[2009]75号);四川省财政基因工程专项资金项目"平菇新品种选育"课题

摘　　要：以姬菇258和金凤2-1为亲本,单孢杂交,获得杂交菌株81株。通过定性出菇表明,其中44号、6号、115号和7号菌株子实体形态具有姬菇的典型特征。通过拮抗反应、ISSR分子标记及聚类分析表明,这4株姬菇新菌株与亲本之间存在遗传差异,证实了杂交姬菇新菌株的真实性;4株新菌株之间也各自保持着个体遗传上的独立和稳定,即构建创制出了4株杂交姬菇新菌株。81 hybrid strains were obtained by single spore crossbreeding between Pleurotus cornucopiae No. 258 and Pleurotus sp. Jin feng2-1. The fruiting test showed that No. 44,No. 6,No. 115 and No. 7 strains＇ fruiting bodies had the characteristic feature of P. cornucopiae.Antagonistic test,ISSR marker test and cluster analysis showed that the four hybrid strains＇ genetic were different from parents and they were different from each other.

关 键 词：姬菇258 金凤2-1 单孢杂交 出菇试验 姬菇特征 拮抗反应 ISSR标记 聚类分析 

分 类 号：S646[农业科学—蔬菜学]