家蚕雌激素相关受体基因的克隆与表达分析  

Molecular cloning and expression profiling of an estrogen-related receptor gene in the silkworm,Bombyx mori

作　　者：沈关望[1] 胡诗圆 王勇[2] 吴金鑫[1] 林英[1] 夏庆友[1] 

机构地区：[1]西南大学家蚕基因组生物学国家重点实验室,重庆400716 [2]西南大学生物技术学院,重庆400716

出　　处：《昆虫学报》2015年第12期1278-1284,共7页Acta Entomologica Sinica

基　　金：国家高技术研究发展计划(“863”计划)项目(2011AA100306);国家重点基础研究发展计划(“973”计划)项目(2012CB114600);国家自然科学基金青年基金项目(31101768,31172157)

摘　　要：【目的】黑腹果蝇Drosophila melanogaster雌激素相关受体（estrogen-related receptor,ERR）通过调节糖酵解过程进而控制果蝇的能量代谢。本研究在克隆家蚕Bombyx mori ERR基因（Bm ERR）的基础上,对其分子特性和系统演化进行生物信息学分析,并检测该基因在家蚕生殖腺中的表达,为进一步研究ERR功能奠定基础。【方法】采用PCR技术克隆Bm ERR基因的全长c DNA序列,进行生物信息学分析;利用半定量RT-PCR检测该基因在停食后家蚕幼虫生殖腺中的表达情况。【结果】Bm ERR基因全长c DNA序列为1 296 bp,编码431个氨基酸残基;具有ERR蛋白家族典型的结构特征;系统进化分析显示Bm ERR与其他昆虫ERR氨基酸序列一致性较高;半定量RT-PCR检测表明,Bm ERR在家蚕上簇到化蛾期间的精巢和卵巢中均有表达,表达具有时期特异性,化蛹第1天达到表达高峰。【结论】本研究首次从鳞翅目昆虫中克隆获得ERR c DNA序列。ERR基因在家蚕生殖腺中表达量无明显性别差异,但具有发育时期特异性。[ Aim] The estrogen-related receptor （ERR） via regulating the glycolytic cyclecan control the energy metabolism in Drosophila melanogaster. Based on the cloning of ERR gene （BmERR） from the silkworm, Bombyx mori, its molecular property and phylogeny were bioinformatically analyzed, and the expression of the ERR gene in the silkworm was detected. This study may lay the foundation for further research on the function of ERR in Lepidoptera and provide ideas for pest control. [ Methods ] PCR technique was used to clone the cDNA sequence of BmERR gene, and the sequence was bioinformatically analyzed with online resources. The semi-quantitative RT-PCR was further applied to analyze its expression profiles in gonads of B. mori larvae stopping feeding. [ Results ] The full-length cDNA of BmERR has a 1 296 bp open reading frame, which encodes a 431-amino-acid-residue protein with a typical structural domain feature of the ERR protein family. Phylogenetic analysis indicated that the BmERR protein shows high amino acid sequence identity with the ERR proteins from other insects. RT-PCR detection showed that BmERR was expressed in both testis and ovary in wandering to pupal B. mori, and time specificity, with a peak expression at the first day after pupation. [ Conclusion ] encoding sequence is reported for the first time in I_epidoptera. The expression of the silkworm gonads has no significant gender difference, but with developmental stage specifi The cloned ERR ERR gene in the city.

关 键 词：家蚕 雌激素相关受体 克隆 序列分析 表达谱 

分 类 号：Q966[生物学—昆虫学]