常温机械灌注条件下劈离式肝移植猪模型的建立  

Establishment of a split liver transplantation pig model using extracorporeal normothermic machine perfusion

作　　者：隋明昊[1] 高伟[2] 沈中阳[2] 

机构地区：[1]天津医科大学第一中心临床学院,天津300192 [2]天津市第一中心医院器官移植中心

出　　处：《中华肝胆外科杂志》2016年第1期48-51,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家高技术研究发展计划（863）项目（2012AA021001）;天津市科技计划项目（12ZCZDSY02600）

摘　　要：目的总结建立常温机械灌注条件下劈离式肝移植模型的经验。方法巴马小型猪20头，供体受体各10头。供肝在常温机械灌注条件下沿Taira线进行劈离，保留右半肝。右半肝在无转流条件下行原位肝移植术。常温机械灌注过程中监测猪门静脉和肝动脉流量、灌注压力和胆汁生成量，取血检测生化和血气分析指标，取肝脏组织行HE染色并观察病理变化。记录受体手术过程中血流动力学变化以及术后5天存活率和死亡原因。结果灌注过程中血流动力学、血生化和血气分析指标保持稳定，肝移植术中无受体死亡。术后第2天死亡2头，术后5天存活率为80％（8／10）。结论在常温机械灌注条件下行肝劈离术，用右半肝在无转流条件下行肝移植术建立大动物模型具有较好的稳定性和重复性，为下一步研究奠定了基础。Objective To summarize the experience of establishing a split liver transplantation pig model using extracorporeal normothermic machine perfusion （NMP）. Methods Twenty miniature pigs were purchased with ten as donors and another ten as receptors. The graft was spliced along Taira line and the right half was reserved for transplantation. Hemodynamics and bile production volume were monitored, and blood bi- ochemical and blood gas analysis indicators were detected during machine perfusion. Pathological change was observed by HE stain. Hemodynamics during liver transplantation, 5-day survival rate and the cause of death were recorded. Results Hemodynamic, biochemical and blood gas analysis indicators remained stable during NMP. All receptor pigs were successfully extubated and awake after surgery. Two receptors died on the second day after the operation. The 5-day survival rate was 80%. Conclusion The split liver transplantation pig model using extracorporeal normothermic machine perfusion is feasible and appropriate, and it lays the founda- tion for further investigation.

关 键 词：劈离式肝移植 猪 常温机械灌注 

分 类 号：R657.3[医药卫生—外科学]