宁肺合剂对慢性阻塞性肺疾病大鼠血TNF-а和IL-8的影响  

Effect of Ningfei Mixture on Blood TNF-а and Interleukin-8 in Rats of Chronic Obstructive Pulmonary Disease

作　　者：于鸿[1] 计忠宇[1] 

机构地区：[1]河北医科大学沧州中西医结合临床医学院,河北沧州061001

出　　处：《四川中医》2016年第1期56-58,共3页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:探讨宁肺合剂对肺脾气虚型和肺肾两虚型慢性阻塞性肺疾病大鼠血TNF-а和IL-8含量的影响。方法:90只健康大鼠按随机数字表法分成正常对照组,肺脾气虚模型组,肺肾两虚模型组,宁肺合剂低、中、高剂量组,采用"烟熏加气管内滴入脂多糖联合番泻叶灌胃泻下法制备肺脾气虚型"和"烟熏加气管内滴入脂多糖联合背部皮下注射琥珀酸氢化可的松复制肺肾两虚型"慢阻肺大鼠模型,实验结束时,观察各组大鼠肺组织病理学改变和血清TNF-а和IL-8含量。结果:模型组大鼠血TNF-а和IL-8含量明显升高,与正常对照组比较有显著差异(P<0.05),宁肺合剂高剂量组、中剂量组和低剂量组与模型组比较血TNF-а和IL-8含量下降(P<0.05)。结论:宁肺合剂可以降低血TNF-а和IL-8含量,减轻慢阻肺气道炎症。Objective： To discuss the effect of Ningfei Mixture to TNF-α and Interleukin-8 （IL-8） in chronic obstructive pulmonary disease （COPD） rat model which were deficiency in lung and spleen and deficiency in lung and kidney. Methods： 90rats were randomly divided into normal group, model groups, low-dose group, medium-dose group and high-dose group. The rat COPD model which was deficiency in lung and spleen was established by intratracheal instillation of Lipopoly saccharide （LPS）, exposure to cigarette smoke and intragastric administration the cool folium sennae soak; the rat COPD model which was deficiency in lung and kidney was established by intratracheal instillation of Lipopoly saccharide （ LPS）, exposure to cigarette smoke and hypodermic injection hydrocortisone. When the test was over, we could observe the pathological changes and the levels of TNF-α and IL-Sin blood serum of every rat in each group. Results. The levels of TNF-α and IL-8were higher in the model groups comparing with the normal group. Comparing with the model groups, the levels of TNF-α and IL-8were lower in Ningfei Mixture groups （P〈0. 05 ）. Conclusion： Ningfei Mixture can reduce the levels of TNF-α, tL-8and airway inflammation in COPD rats.

关 键 词：慢性阻塞性肺疾病 宁肺合剂 肺脾气虚 肺肾两虚 TNF-а IL-8 

分 类 号：R285.5[医药卫生—中药学]