苦豆子植株不同部位醇提液对大鼠离体小肠运动的影响  

Effect of Alcohol Extract from Different Parts of Sophora alopecuroides on the Motility of Isolated Rat Small Intestine

作　　者：张丹[1] 石刚[2] 周玉东[1] 闫静[1] 王丽芳[1] 孟坤杰 郭晶[1] 赵红琼[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]新疆畜牧科学院畜牧研究所,乌鲁木齐830000

出　　处：《中国畜牧兽医》2016年第1期164-170,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家青年科学基金(31201872);新疆自治区级大学生创新项目(10755201400041)

摘　　要：为比较秋季苦豆子植株不同部位提取物对消化道运动的干预作用,本研究利用生物信号处理系统测定了浓度递增（0.5%~8.0%）的苦豆子植株不同部位（豆子、豆荚、叶和顶须）醇提液对大鼠离体小肠平滑肌运动的影响,并初步探究其机理。结果显示,与用药前相比,苦豆子植株各部位均在浓度递增到2.0%~4.0%时使离体小肠收缩频率和幅度减小（P〈0.05）,并呈剂量依赖关系,而对收缩张力影响不显著（P〉0.05）,其中豆子抑制作用最强。同时,胆碱能M-受体激动剂氯贝胆碱引起的小肠平滑肌兴奋作用可被2.0%豆子完全抑制,且2.0%豆子的抑制作用不能被氯贝胆碱扭转。结果表明,苦豆子植株各部位对离体小肠运动均有抑制作用,且可能与阻断M-受体作用有关。提示苦豆子植株各部位有望用于缓解由于某些原因引起的胃肠活动过度症状。To explore the effect of extract from different parts of Sophora alopecuroides picked in autumn on gastrointestinal motility,alcohol extract of bean,pod,leaf and shoot apex of Sophora alopecuroides at increasing dose（0.5%to 8.0%）were added to Tyrode＇s solution where rat isolated small intestine were bathed.The motility of small intestine was recorded by biological signal processing system.The results showed that the contract frequency and amplitude were significantly decreased when the concentration of each parts of Sophora alopecuroides reached 2.0%to4.0% compared with that before treatment（P〈0.05）and it was the dose dependent relation ship,but had no significant effect on the contract tension（P〉0.05）.Moreover,the inhibition effect of bean was the strongest among the four parts.Alcohol extract of bean at a dose of 2.0%inhibited the excitatory effect caused by cholinergic M-agonists,bethanechol,and the inhibition couldn＇t be reversed.The results concluded that different parts of Sophora alopecuroides could inhibit the contraction of rat small intestine,and the inhibition might happen partly by blocked Mreceptor,indicating that Sophora alopecuroides are expected to be used to relieve gastrointestinal over contraction due to some reasons.

关 键 词：苦豆子 小肠 离体 平滑肌 收缩性 大鼠 

分 类 号：S853.7[农业科学—临床兽医学]