鸭致病性大肠杆菌分离鉴定及其相关致病性分析  

Isolation,Identification and Related Pathogenicity Analysis of Duck Pathogenic E.coli

作　　者：邓伯雄 刘情[1] 邓安元[1] 李文文[1] 宋志刚[1] 余琼[2] 陈光芬 刘述皇 刘亚刚[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]西昌市畜牧局,西昌615000 [3]西昌市华宁农牧科技有限公司,西昌615000 [4]云南大理市畜牧工作站,大理671003

出　　处：《中国畜牧兽医》2016年第1期235-241,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家农业科技成果转化资金项目--国家科技部农业项目"规模化养鸭场标准化健康养殖技术示范与推广"(2013GB2F000422)

摘　　要：为研究鸭大肠杆菌的致病性及相关生物特性,本试验从西昌市某规模化鸭场采集病料,通过传统分离培养及理化性质鉴定,分离得到27株鸭致病性大肠杆菌。对27株鸭致病性大肠杆菌进行血清型鉴定、药敏试验、相关毒力基因检测。血清型鉴定结果显示,优势血清型为O119、O86、O126、O142和O44,占分离株55.56%。血清型O119占27株鸭致病性大肠杆菌的40.74%,为该鸭场流行的致病血清型。对20种兽医临床常用药物的药敏试验结果显示,27株鸭致病性大肠杆菌均对阿米卡星、庆大霉素和多黏菌素B敏感,对头孢曲松等10种药物较敏感,对利福平等5种药物耐受。大肠杆菌相关毒力基因检测结果显示,iutA、hlyF、Iss、IroN、ompT、fyuA、irp2、Tsh及papA基因携带率均为100.00%,fimC基因携带率59.26%,K99基因携带率7.40%。各项研究结果为有效防控鸭大肠杆菌病提供了重要科学依据,并为大肠杆菌深入研究奠定了基础。To analyze the pathogenicity and biological characteristics of duck E.coli,27 strains of duck pathogenic E.coli were isolated from dead ducks with the typical characterize of colibacillosis by isolation and culture,physical and chemical properties identification in Xichang city.O serotype identification,drug sensitivity test and virulence associated genes were detected in 27 strains of duck pathogenic E.coli.The result showed that the predominant serotypes were O119,O86,O126,O142 and O44,which accounted for 55.56%.O119 was the epidemic and pathogenic serotype in this farm,which accounted for 40.74%in 27 strains of duck pathogenic E.coli.Through the drug sensitivity test of 20 kinds of clinical commonly used drugs,we found that all strains were sensitive to amikacin,gentamicin and polymyxin B.Besides,10drugs（ceftriaxone,etc）were lightly sensitive.However,these strains were resistant to 5kinds of drugs（rifampicin,etc）.The detection result of PCR about virulence associated genes indicated that the positive of iutA,hlyF,Iss,IroN,ompT,fyuA,irp2,Tsh and papA genes were 100.00%,fimCand K99 genes were59.26% and 7.40%,respectively.The results provided an important reference for effective prevention and control of duck colibacillosis,and laid the foundation for further study of E.coli.

关 键 词：鸭致病性大肠杆菌 分离鉴定 耐药性 致病性 

分 类 号：S852.614[农业科学—基础兽医学]