有氧和力竭游泳运动对DNA疫苗免疫效果的影响及机制研究  

Influence and Mechanism of Aerobic and Exhausted Swimming Exercise on Immune Responses of DNA Vaccine

作　　者：宋红利[1,2] 牛英鹏[2] 王军朋[1,3,4] 

机构地区：[1]河南大学淮河临床学院转化医学中心,河南开封475000 [2]河南大学体育学院,河南开封475000 [3]河南大学淮河医院转化医学中心,河南开封475000 [4]河南大学分子医学研究所,河南开封475000

出　　处：《河南大学学报（自然科学版）》2016年第1期76-83,共8页Journal of Henan University:Natural Science

基　　金：河南大学博士启动基金;河南大学种子基金;国家自然科学青年基金(81402677)联合资助

摘　　要：将48只雌性C57BL/6小鼠随机分成对照组(16只),有氧游泳运动组(16只)和力竭游泳运动组(16只).在小鼠分别进行6周有氧和力竭游泳运动后,肌肉注射DNA疫苗3次,每次间隔14d.末次免疫后14d,处死小鼠,收集血液和脾细胞,检测抗原特异性的体液和细胞免疫反应.为了进一步研究不同强度有氧运动影响疫苗的免疫效果的作用机理,小鼠运动6周后,处死小鼠,收集脾细胞分析CD4^+CD25^+调节性T细胞(Treg)的数量及其抑制功能和IL-2的变化.结果显示:和对照组比,有氧运动增强了抗原特异性的细胞免疫反应和细胞毒T淋巴细胞反应(CTL);与之相反,力竭运动抑制抗原特异性的细胞免疫反应和CTL反应.但两种不同强度的游泳运动都不影响血清中抗体的产生.机理研究发现,力竭运动增加Treg细胞的数量及抑制功能,而有氧运动并不影响Treg的数量和功能,但增加脾细胞IL-2的表达.以上结果表明:1)有氧和力竭运动不影响DNA疫苗的体液免疫效果;2)有氧运动可能通过增加脾细胞中IL-2的产生而增强DNA疫苗的细胞免疫效果;3)力竭游泳运动可能通过改变Treg的数量及其抑制功能而削弱DNA疫苗的细胞免疫效果.Forty-eight female C57BL/6mouse were randomly divided into three groups（16mouse/group）：control,aerobic（AE）,and exhausted（EE）swimming exercise groups.To evaluate the effects of aerobic and exhausted exercise on DNA vaccine,the humoral and cellular immune responses were determined in the swimming mice immunized with DNA vaccine for three times.In addition,to elucidate how AE/EE enhance/impair the immune response in a swimming mouse model,the frequency of CD4^＋CD25^＋regulatory T cells（Treg）and the production of IL-2from spleen cells were determined by flow cytometry assay after the completion of six weeks of swimming exercise of mice.Here our data show that,compared with control group,aerobic swimming exercise could augment cell mediated immunities（CMI）such as increasing IFN-γexpression,T cell proliferation and antigen-specific cytotoxic T lymphocyte（CTL）responses in the animal immunized with HBV DNA vaccine.In contrast,the CMI and CTL responses were impaired by exhausted swimming exercises.In addition,both aerobic and exhaustedswimming exercise did not affect antigen-specific IgG levels from serum.Further mechanism study showed that exhausted swimming exercise increased the frequency and inhibitory function of Treg cells;whereas,aerobic exercise did not affect Treg cell frequency and function but increase the IL-2production.These data indicate that 1）both aerobic and exhausted swimming exercise can not affect the humoral response,2）aerobic exercise has the ability to enhance the cellular immune responses of DNA vaccines possibly via increasing the IL-2production of splenocytes,and 3）exhausted exercise can inhibit the cellular immune responses of DNA vaccines possibly via enhancing the number and inhibitory function of Treg cells.

关 键 词：有氧运动 力竭运动 DNA疫苗 细胞免疫 CD4+CD25+调节性T细胞 CTL 

分 类 号：R392.9[医药卫生—免疫学]