PEG胁迫对小麦代换系种子萌发特性的影响及染色体效应  

Effect of PEG Stress on Germination Characteristics and Chromosome Control in Wheat Substitutions Lines

作　　者：薛梦瑶 路斌[2] 杜欢[3] 白志英[3] 

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]河北农业大学园林与旅游学院,河北保定071001 [3]河北农业大学生命科学学院,河北保定071001

出　　处：《安徽农业科学》2015年第36期209-211,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省自然科学基金项目(C2011204016;C2015204066)

摘　　要：[目的]明确干旱胁迫对中国春-Synthetic 6x代换系种子萌发特性的影响并对相关基因进行染色体定位,为选择抗旱高产品系提供理论依据。[方法]以小麦中国春-Synthetic 6x染色体代换系及其亲本为材料,设置对照(无离子水培养)和聚乙二醇(PEG)-6000模拟水分胁迫2个处理,通过测定种子的发芽率、发芽势、发芽指数研究干旱胁迫对种子萌发特性的影响及其染色体调控效应。[结果]干旱胁迫下,中国春-Synthetic 6x各代换系的发芽率、发芽势、发芽指数较对照普遍降低;5B、6B、4D、6D和7D代换系发芽率、发芽势、发芽指数均显著或极显著高于母本中国春。[结论]Synthetic 6x的5B、6B、4D、6D和7D染色体上可能存在干旱胁迫下提高种子活力、促进种子萌发的基因,可在抗旱育种中加以利用。[ Objective] To clear effect of PEG stress on germination characteristics and chromosome control in wheat substitutions lines and locate chromosome of related genes so as to provide theoretical basis for select drought-resistance high yield lines. [ Method] We used Chinese Spring（CS）-Synthetic 6x substitutions lines and parents cultivars ＂Chinese Spring＂ and Synthetic 6x as materials to study the effect on seed germination characteristics and chromosomal effect by measuring germination percentage （GP）, germinating energy （GE） and germination in- dex （GI） under demonized whter （control） and Polyethylene glycol （PEG） -6000 employed as osmotic for simulating water stress. [ Result ] The GP, GE and GI of CS-Synthetic 6x substitutions lines and parents decreased compared to the control conditions under the PEG water- stress. GP, GE and GI of 5B, 6B, 4D, 6D and 7D substitutions lines were significantly higher than those of CS. [ Conclusion] The genes of stimulating germination may be located on 5B, 6B, 4D, 6D and 7D Chromosomes of Synthetic 6x, and they can be used in breeding for drought resistance.

关 键 词：小麦代换系 PEG胁迫 发芽势 发芽率 发芽指数 

分 类 号：S503.4[农业科学—作物学]