有氧运动对胰岛素抵抗小鼠visfatin表达的影响  

Effects of aerobic exercise on the expression of visfatin in mice with insulin resistance

作　　者：李晶[1] 董荣娜[1] 于德民[1] 

机构地区：[1]天津医科大学代谢病医院内分泌研究所,卫生部激素与发育重点实验室,300070

出　　处：《国际内分泌代谢杂志》2016年第1期10-13,31,共5页International Journal of Endocrinology and Metabolism

摘　　要：目的探讨高脂饮食诱导的胰岛素抵抗（IR）小鼠visfatin表达的改变及有氧运动对visfatin表达及脂代谢的影响。方法雄性C57BL／6小鼠24只，按随机数字表随机分为普通饮食组（n=12）和高脂饮食组（n=12），后者喂养8周高脂饲料建立IR模型，再各自分为普通饮食对照组（n=6）、普通饮食运动组（n=6）、高脂饮食对照组（n=6）、高脂饮食运动组（n=6）。两个运动组进行6周跑台训练，各组饮食不变。14周后ELISA检测小鼠血清visfatin，Western印迹检测腓肠肌、肝脏及睾周脂肪visfatin表达。结果8周后，与普通饮食组相比，高脂饮食组空腹胰岛素水平升高，血糖曲线下面积增加（t=6．32，17．44，P均〈0．01）。14周后，高脂饮食运动组血清visfatin水平低于高脂饮食对照组，而普通饮食运动组血清visfatin水平高于普通饮食对照组（F=43．59，P〈0．05）。高脂饮食运动组骨骼肌、肝脏、内脏脂肪组织中visfatin蛋白表达水平较高脂饮食对照组降低（F=29．33—85．17，P均〈0．05），普通饮食运动组骨骼肌visfatin表达较普通饮食对照组升高（F=52．79，P〈0．05），但肝脏、内脏脂肪组织visfatin蛋白表达在普通饮食组间无明显变化（P均〉0．05）。结论高脂饮食可以增加IR模型动物visfatin水平，有氧运动能有效下调visfatin的表达，改善脂代谢紊乱。Objective To explore the expression of visfatin in mice with insulin resistance （IR） induced by high-fat diet and the effects of aerobic exercise on the expression of visfatin and lipid metabolism. Methods A total of 24 male C57BL/6 mice were randomly divided into normal diet group （n = 12 ） and high-fat diet group （n = 12） according to the table of random number. Mice in high-fat diet group were fed with high-fat diet for 8 weeks to induce IR. Both groups were divided into normal diet control group （ n = 6） , normal diet exercise group（ n = 6）and high-fat diet control group （n = 6）, high-fat diet exercise group （n = 6 ） , respectively. Mice in both exercise groups underwent treadmill exercise for 6 weeks. All mice did not change diet. After 14 weeks, serum visfatin concentration was evaluated by ELISA. And Western blotting was applied to detect the expression of visfatin in gastrocnemius muscle, liver and epididymal tissue. Results Eight weeks later, compared with normal diet group, fasting insulin of high-fat diet group was elevated, and blood glucose area under curve was increased （ t = 6.32,17.44, all P 〈 0.01 ）. Fourteen weeks later, the level of serum visfatin in high-fat diet exercise group was lower than that in high-fat diet control group, but the level of serum visfatin in normal diet exercise group was higher than that in normal diet control group （F = 43.59, P 〈 0.05 ）. Compared with high-fat diet control group, the expression of visfatin in skeletal muscle, liver and adipose tissue were reduced in high-fat diet exercise group （ F = 29.33-85.17, all P 〈 0.05 ）. On the contrary,the expression of visfatin in skeletal muscle in normal diet exercise group was higher than that in normal diet control group（ F = 52.79, P 〈 0.05 ） , but there were no differences in the expression of visfatin in liver and adipose tissue between normal diet groups （ all P 〉 0.05 ）. Conclusion High-fat diet increases the level of visfatin, whereas aerobic exercise reduce

关 键 词：有氧运动 胰岛素抵抗 内脂素 脂代谢 

分 类 号：R378.2[医药卫生—病原生物学]