冠心平对麻醉犬冠脉结扎所致急性心肌缺血的影响  

Effect of Guanxinping on Anesthetic Dog with Acute Myocardial Ischemia Caused by Coronary Artery Ligation

作　　者：李芳[1,2] 杨成流 李卓琼[1,2] 周军[1,2] 毕宇安[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国中医急症》2016年第1期14-16,36,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：科学技术部国家重大新药创制项目(2013ZX090402203)

摘　　要：目的观察冠心平对麻醉犬冠脉结扎所致心肌缺血的影响。方法采用麻醉犬冠状动脉左前降支结扎造成急性心肌缺血模型,将36只Beagle犬随机分为假手术组、模型组、心可舒组及冠心平高、中、低剂量组,观察心外膜电图、检测血氧及进行组织病理学检查。结果冠状动脉结扎后,各组心肌缺血犬的∑-ST显著增加,与假手术组比较有明显差异(P<0.01)。模型组给予生理盐水后,∑-ST无明显影响,与假手术组比较仍有显著性差异(P<0.01)。给予冠心平后,冠心平高、低剂量组与模型对照组比较,在给药60、120、180 min时∑-ST有显著降低(P<0.05或P<0.01),冠心平中剂量在给药120、180 min时∑-ST有显著降低(P<0.01)。冠状动脉结扎后,各组心肌缺血犬的N-ST较假手术组明显增加(P<0.01)。模型组给予生理盐水后,N-ST无明显影响,与假手术组比较仍具有显著性差异(P<0.01)。给予冠心平后,冠心平高、中、低剂量组在给药60、120、180 min时较模型组N-ST明显降低(P<0.05或P<0.01)。冠状动脉结扎后,各组心肌氧利用率呈下降趋势,但与假手术组比较无显著差异(P>0.05)。给药3 h与给药前比较,心肌氧利用率呈上升趋势,冠心平高、中剂量组给药后3 h与给药前比较有显著差异(P<0.01)。假手术组心肌细胞横纹清晰,核居中,未见变性坏死;间质未见血管扩张及炎细胞浸润及纤维组织增生。模型组主要表现心肌部分区域见心肌细胞小片状或小灶性坏死,表现为细胞轮廓尚可见,但胞浆红染、心肌纤维水肿溶解断裂、胞核固缩碎裂或消失;心肌间质水肿、内有多量炎细胞浸润(中性粒细胞、淋巴细胞和浆细胞),个别坏死区附近可见肉芽组织以及增生的纤维结缔组织。各给药组心肌细胞未见坏死、部分区域可见心肌纤维水肿,溶解断裂;间质水肿,有少许炎细胞浸润和纤维组织增生。冠心平各剂量组较模型组病变均有改善,�Objective： To study the effect of Guanxinping on anesthetic dog with myocardial ischemia caused by coronary artery ligation. Methods： The acute myocardial ischemic canine models were established by left anterior descending artery ligature after opening thoracic cavity under anesthesia. 36 Beagle dogs were randomly assigned to six groups,which were the sham operation group,the model group,Xinkeshu group,and Guanxinping groups with high,median,and low doses of the drugs. The epicardial electrical diagram and blood oxygen were observed,and histopathological examination was evaluated. Results： The range and the degree of myocardial ischemia,as well as the pathogenic change of myocardium in the Guanxinping groups were significantly lower than that of the model group. Moreover,myocardial oxygen utilization rate of 3 h after the treatment rised obviously. Conclusion：Guanxinping has obvious protective effect on acute myocardial ischemiain dogs.

关 键 词：冠状动脉结扎 心肌缺血 冠心平 麻醉犬 

分 类 号：R285.5[医药卫生—中药学]