调心方对阿尔茨海默病模型鼠学习记忆能力及p62表达水平的影响  

Effect of Tiaoxin Fang on Learning and Memory Ability and P62 Expression Level of Alzheimer Disease Model Mice

作　　者：邢三丽[1] 申定珠[1] 陈川[1] 郁志华[1] 吴蓓玲[1] 迟惠英[1] 

机构地区：[1]上海市中医老年医学研究所,上海200031

出　　处：《中国中医急症》2016年第1期45-47,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：上海市自然科学基金项目(13ZR1439800)

摘　　要：目的研究调心方对阿尔茨海默病模型小鼠学习记忆功能的影响及其与自噬受体蛋白p62相关的作用机制。方法采用24周龄APP/PS1双转基因小鼠作为早中期阿尔茨海默病模型动物,同时给予调心方及多奈哌齐治疗共12周,采用Morris水迷宫检测各组小鼠学习记忆能力以及用免疫荧光染色、Western blotting法检测小鼠海马区域p62蛋白的表达。结果与正常对照组比较,APP/PS1模型组小鼠学习记忆能力受损,海马区域p62表达降低,经中药调心方治疗后,能明显改善APP/PS1小鼠的学习记忆能力,提高大脑海马区域p62的表达。结论调心方可通过提高海马区域p62的表达而改善学习记忆功能。Objective： To investigate the effect of Tiaoxin Fang,a compound drug of traditional Chinese medicine,on learning and memory ability and its mechanism related to autophagic receptor p62 in Alzheimer′ s disease model mice. Methods： The 12 weeks′ old APP/PS1 transgenic mice were selected as early stage of Alzheimer′s disease. Meanwhile,they were treated with Tiaoxin Fang by gastrogavage for 12 weeks. The learning and memory ability was measured with Morris water maze. The protein expressions of p62 in hippocampus of mice were detected by immunofluorescence staining and Western blot assay. Results： Compared with the control group,the learning and memory ability of APP/PS1 mice were damaged,and the expression of p62 in hippocampus decreased. After administrating Tiaoxin Fang,the learning and memory ability and p62 expression of APP/PS1 mice were significantly improved. Conclusion： Tiaoxin Fang ameliorates the learning and memory impairment by improving the expression of p62 in the hippocampus.

关 键 词：阿尔茨海默病 调心方 P62 

分 类 号：R285.5[医药卫生—中药学]