汽车尾气来源PM2.5颗粒物长期暴露导致SD雄鼠生殖功能损害  

Long-term exposure to PM2.5 from automobile exhaust results in reproductive dysfunction in male rats

作　　者：严超[1] 曹希宁[1] 沈炼桔[1] 刘东尧[1] 彭金普 陈进军[1] 周玥[1] 龙春兰[1] 林涛[1] 何大维[1] 李旭良[1] 魏光辉[1] 

机构地区：[1]重庆医科大学附属儿童医院泌尿外科儿科学重庆市重点实验室儿童发育疾病研究教育部重点实验室,重庆400014

出　　处：《中华男科学杂志》2016年第2期104-109,共6页National Journal of Andrology

基　　金：国家自然科学基金(81370701;31200853);重庆市博士后资助项目(Xm2014040);重庆市科委重点实验室专项经费;国家临床重点专科建设项目(国卫办医函[2013]544);重庆市高校创新团队建设计划项目;重庆市科技创新领军人才项目~~

摘　　要：目的：建立Sprague Dawley（SD）大鼠空气细颗粒物（PM2.5）长期暴露模型,研究PM2.5长期暴露对SD大鼠生殖功能的损伤及其机制。方法：45只SD大鼠（80~94 g,28日龄）随机分配成3组：生理盐水对照组,低剂量组[2μg/（100 g·d）],高剂量组[16μg/（100 g·d）]。每天分别给予0.9%的生理盐水或PM2.5颗粒,连续暴露60 d,最后一次暴露24 h后各实验组选取10只与正常雌鼠合笼,统计雌鼠受孕率,剩余实验鼠取材,进行精子计数和畸形率测定、睾丸组织病理学检测以及睾丸组织Connexin43蛋白表达检测。结果：与对照组雌鼠受孕率（100%）相比,PM2.5暴露组正常雌鼠受孕率（高剂量组50%,低剂量组70%）明显降低,精子数量和质量明显下降,生精小管结构紊乱,管腔精子数减少,部分次级精母细胞脱落至管腔。睾丸组织中Connexin43蛋白表达下降,血睾屏障破坏。结论：汽车尾气来源PM2.5长期暴露影响SD大鼠生殖功能。Objective ： To explore the effects of long-term exposure to particulate matter 2.5 （ PM2.5 ） from automobile exhaust on the reproductive function of Sprague Dawley （SD） rats. Methods ： Forty-five male SD rats, weighing 80 - 94 g and aged 28 days, were randomly assigned to receive intra-tracheal administration of 0.9% normal saline （control group, n = 15 ）, PM2.5 at 2 μg per 100 g body weight per day （low-dose PM2.5 group, n = 15）, and PM2.5 at 16 μg per 100 g body weight per day （high-dose PM2.5 group, n = 15） , qd, for 60 successive days. After the last 24-hour exposure, 10 rats were taken from each group for copulation with normal female ones, while the others were sacrificed, their testes removed for sperm count and deformity, pathological examination, and determination of the Connexin43 expression. Results ： The conception rate was significantly decreased in the low- and high-dose PM2.5 groups as compared with that of the control （70% and 50% vs 100% ）, and so were the sperm count and quality. The rats in the PM2.5-exposed groups showed significantly disordered histological structure of the seminiferous tubules, reduced sperm count in the testicular lumen, some exfoliated secondary spermatocytes, downregulated Connexin43 expression in the testis, and damaged blood-tes- tis barrier. Conclusion ： Long-term exposure to PM2.5 from automobile exhaust damages the reproductive function of male SD rats.

关 键 词：空气细颗粒物 大鼠 汽车尾气 生殖功能 

分 类 号：R114[医药卫生—卫生毒理学] X513[医药卫生—公共卫生与预防医学]