重组人促红细胞生成素对感染致肝肾损伤及内质网应激的保护作用  

Protective effect of recombinant human erythropoietin on liver and kidney injury as well as endoplasmic reticulum stress caused by infection

作　　者：江燕丽 姬生威[1] 苏梅芳[1] 刘静[1] 

机构地区：[1]湖北省黄冈市中心医院,湖北黄冈438000

出　　处：《海南医学院学报》2016年第5期424-427,共4页Journal of Hainan Medical University

基　　金：2012年黄冈市科技局项目(EK2013D210014000788)~~

摘　　要：目的:研究重组人促红细胞生成素(rHuEPO)对感染引起的肝肾损伤以及内质网应激的保护效果。方法:将雄性Wistar大鼠随机分为阴性对照组、脂多糖(LPS)组和LPS+rHuEPO组(n=30)。LPS组和LPS+rHuEPO组采用尾静脉注射LPS的方法建立感染模型;LPS+rHuEPO组采用尾静脉注射rHuEPO的方法进行干预。干预前和干预后6、12h时,采集血清检测TNF-α、iNOS、BUN、Cr、ALT、AST水平;干预后12h时,采集肝肾组织,检测内质网应激分子(GRP78、CHOP、Caspase-12)的mRNA水平。结果:建模后、rHuEPO干预前,LPS组和LPS+rHuEPO组大鼠血清中TNF-α、iNOS、BUN、Cr、ALT、AST水平均高于阴性对照组,但两组间无显著差异;干预后6、12h时,LPS+rHuEPO组大鼠血清中TNF-α、iNOS、BUN、Cr、ALT、AST水平均低于LPS组;干预后12h时,LPS+rHuEPO组大鼠肝、肾组织中GRP78、CHOP、Caspase-12的mRNA水平低于LPS组。结论:大鼠LPS感染模型中,rHuEPO对感染所致肝肾损伤和内质网应激具有保护作用。Objective： To study the protective effect of recombinant human erythropoietin on liver and kidney injury as well as endoplasmic retieulum stress caused by infection. Methods： Male Wistar rats were randomly divided into negative con- trol group, LPS group and LPS4-rHuEPO group, LPS group and LPS rHuEPO group received injection of LPS via caudal vein to establish infection models, and LPS--rHuEPO group received injection of rHuEPO via caudal vein for intervention; be- fore intervention as well as 6 h and 12 h after intervention, serum was collected to detect TNF-α, iNOS, BUN, Cr, ALT and AST contents; 12 h after intervention, liver and kidney tissue was collected to detect mRNA contents of endoplasmic reticulum stress molecules （GRP78, CHOP and Caspase-12）. Results： After model establishment and before rHuEPO intervention, ser- um TNF-α, iNOS, BUN, Cr, ALT and AST contents of LPS group and LPSrHuEPO group had no differences and were higher than those of negative control group; 6h and 12 h after intervention, serum TNF-α, iNOS, BUN, Cr, ALT and AST contents of LPS-4-rHuEPO group were lower than those of LPS group; 12 h after intervention, mRNA contents of GRP78,CHOP and Caspase-12 in liver and kidney tissue of LPS rHuEPO group were lower than those of LPS group. Conclusion： Recombinant human erythropoietin has protective effect on liver and kidney injury as well as endoplasmic reticulum stress caused by infection.

关 键 词：促红细胞生成素 保护 肝功能 肾功能 内质网应激 

分 类 号：R631[医药卫生—外科学]