急性胰腺炎胰腺白细胞、血清白细胞介素-6、腹水胰淀粉酶变化的意义  

Significance of white blood cells, serum interleukin - 6 and ascites pancreatic amylase in acute pancreatifis

作　　者：孙亮[1] 曾玉剑[1] 罗华友[1] 王昆华[1] 李立[2] 

机构地区：[1]昆明医科大学第一附属医院胃肠外科,650032 [2]昆明医科大学附属甘美医院肝胆胰一科,650011

出　　处：《中华实验外科杂志》2016年第1期43-45,共3页Chinese Journal of Experimental Surgery

基　　金：云南省科技厅基金（2015FB024）

摘　　要：目的观察急性胰腺炎（AP）胰腺组织白细胞、血清白细胞介素（IL）-6、腹水胰淀粉酶（AMS）的变化及意义。方法40只SD大鼠随机分对照组、AP3h组、AP6h组和AP12h组，并用扫描电镜检测胰腺组织白细胞的变化，用酶联免疫吸附试验（ELISA）检测血清IL-6的蛋白变化及腹水胰淀粉酶的动态变化。结果对照组胰腺组织可见少量白细胞[（2．00±0．71）个]，细胞结构完整，此外还可见大量胶原纤维。AP3h白细胞数量[（5．80±0．84）个]明显增多（P〈0．01）。AP6h自细胞数量[（3．60±1．14）个]有所下降（P〈0．05），细胞伪足明显减少，胶原纤维数量减少、排列紊乱。AP12h可见大量红细胞散在分布，白细胞和胶原纤维明显崩解，白细胞数量[（0．60±0．55）个]急剧下降，明显低于对照组（P〈0．05）、AP3h组（P〈0．01）和AP6h组（P〈0．05）。AP后3h[（756．10±28．64）ng／L，P〈0．01]、6h[（1010．90±3．62）ng／L，P〈0．01]和12h[（555．51±0．85）ng／L，P〈0．01]血清IL-6表达水平明显高于对照组，AP6h表达最高，明显高于AP3h（P〈0．05）和AP12h（P〈0．05）。AP后3hI（1169．90±50．88）U／dl，P〈0，01）、6h[（1069．90±24．15）U／dl，P〈0．01]和12h[（1068．40±17．28）U／dl，P〈0．01]腹水AMS活力值均明显高于对照组。AP3h腹水胰淀粉酶活性活力值最高，明显高于AP6h（P〉0．05）和12h（P〉0．05），但6h和12h比较差异无统计学意义（P〉0．05）。结论胰腺白细胞数量、血清IL-6、腹水淀粉酶的变化与水肿型AP向坏死型AP发展密切相关。Objective Study significance of white blood cells, serum interleukin （IL） -6 and ascites amylase in acute pancreatits. Methods 40 SD rats were randomly divided into control group, AP 3 h group, AP 6 h group and AP 12 h group.Changes of white blood ceils of pancreas were detected by scanning electron microscope, and changes of IL - 6 protein in serum was detected by enzyme linked immunosorbent assay （ELISA）. Also, ascites pancreatic amylase activity was detected by amylase activity kit. Results There was a small amount of white blood cells （WBC） in pancreatic tissue, and a large number of collagen fibers in control group （ 2. 00 ± 0. 71 ）. Count of WBC （ 5. 80 ± 0. 84） was significantly increased at AP 3 h （P 〈 0. 01 ）. Then the count （3.60 ± 1.14） was decreased at AP 6 h （P 〈 0. 05 ）, the number of pseudopods and collagen fibers were also decreased. A large number of red blood cells instead of WBC, collagen fibers and WBC both were disintegration at AP 12 h. The number of WBC （0. 60 ±0. 55） was significantly lower than that in control group （P 〈0. 05）, AP 3 h group （P 〈0. 01 ） and AP 6 h group （P 〈 0. 05）. Levels of serum IL - 6 was significantly higher compared with that in AP 3 h [ （756. 10 ± 28.64） ng/L, P〈0.01], 6 h [（1 010.90±3.62） ng/L,P〈0.01], and 12 h [（555.51 ±0.85） ng/L, P 〈0. 01 ]. And the level of IL-6 reached the peak at AP 6 h. Ascites pancreatic amylase value was the highest at AP 3 h （1 169. 90± 50. 88） U/dl compared with control group （P〈 0.01）, AP 6 h [ （ 1 069. 90 ±24. 15） U/dl and 12 h （ 1 068. 40 ± 17. 28） U/dl]. However, there was not statistically significant between 6 h and 12 h （ P 〉 0. 05 ）. Conclusion The number of WBC in pancreas, level of serum IL- 6 and ascites pancreatic amylase have a close relationship with the process in edematous to necrotizing AP.

关 键 词：急性胰腺炎 电镜 白细胞 白细胞介素-6 腹水胰淀粉酶 

分 类 号：R135.12[医药卫生—劳动卫生]