腺病毒携带骨形成蛋白7基因对小鼠膝关节假体松动模型假体周围组织炎性反应的影响  

Effects of recombinant adenovirus carrying bone morphogenetic protein 7 gene on inflammatory response in periprosthetic tissue of mice knee prosthesis loosening model

作　　者：吴延平[1,2] 贾广红 焦兆德[2] 夏长所[1] 王英振[1] 

机构地区：[1]青岛大学医学院关节外科,266101 [2]潍坊市益都中心医院关节外科,261000

出　　处：《中华实验外科杂志》2016年第1期188-191,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察腺病毒携带骨形成蛋白7（BMP-7）基因对小鼠膝关节假体松动模型假体周围组织炎性反应的影响。方法120只8周龄无特定病原体（SPF）级BABL／c雌性小鼠随机分为空白对照组、阳性对照组和BMP-7干预组，构建膝关节假体松动模型。检测小鼠假体周围组织BMP-7 mRNA和核因子（NF）-κB mRNA表达，并测定炎性因子水平。结果与空白对照组和阳性对照组比较，BMPG干预组T2-T4时小鼠假体周围组织中BMP-7 mRNA相对表达量（3．61±0．29、5．03±0．41和6．14±0．38）均升高（P〈0．05）；与空白对照组和阳性对照组比较，BMPM干预组T1～T4时小鼠假体周围组织中白细胞介素（IL）-1β[（134．7±9．8）、（153．5±14．2）、（169．4±12．7）、（178．6±11．3）ng/L]、IL-6[（66．2±8．4）、（77．6±7．3）、（91．4±15．2）、（107．2±11．5）ng／L]、肿瘤坏死因子（TNF）-α[（79．5±10．6）、（96．2±12．4）、（118．3±14．1）、（133．7±13．2）ng/L]和NF—κB mRNA（1．59±0．22、2．05±0．19、2．67±0．25和3．04±0．23）均降低（P〈0．05）。结论腺病毒携带BMPG基因可在小鼠膝关节假体松动模型假体周围组织内表达，可抑制假体周围炎性因子的释放。Objective To investigate the effects of recombinant adenovirus carrying bone morphogenetic protein 7 （ BMP - 7） gene on inflammatory response in periprosthetic tissue of mice knee prosthesis loosening model. Methods 120 cases of 8 week - old specific pathogen free （SPF） BABL/c female mice were randomly divided into control group, positive control group and BMP - 7 intervention group （ n = 40 each）. Knee prosthesis loosening models were constructed. The mRNA expression of BMP- 7 and nuclear factor - κB （NF - κB） was detected. The inflammatory cytokine levels were measured. Results Compared with the control group and the positive control group, the relative expression levels of BMP - 7 mRNA （3.61 ±0. 29, 5.03 ±0. 41 and 6. 14 ±0. 38） in the BMP -7 intervention group at T2 -T4 were increased （P 〈 0. 05 ）. Compared with the control group and the positive control group, the levels of interleukin （IL） -1β [（134.7 ±9.8）, （153.5 ± 14.2）, （169.4 ± 12.7）, （178.6 ± 11.3） ng/L], IL -6 [（66.2±8.4）, （77.6 ±7.3）, （91.4 ± 15.2）, （107.2 ± 11.5） ng/L], tumor necrosis factor （TNF） -α [（79.5 ±10.6）, （96.2 ±12.4）, （118.3 ±14.1）, （133.7 ±13.2） ng/L] andNF-κB mRNA （ 1.59 ± 0. 22, 2. 05 ± 0. 19, 2. 67 ± 0. 25, 3.04 ± 0. 23 ） of periprosthetic tissues at T2 - T4 in the BMP - 7 intervention group were significantly decreased （ P 〈 0. 05 ）. Conclusion Adenoviral - mediated BMP - 7 gene could be expressed in the periprosthetic tissues of the mice knee prosthesis loosening model. It could inhibit the inflammatory eytokine release around the prosthesis.

关 键 词：腺病毒 骨形成蛋白7 炎性因子 

分 类 号：R456[医药卫生—治疗学]