溃疡性结肠炎患者中维生素D受体FokI基因多态性与维生素D水平的关系  

An analysis of vitamin D receptor FokI gene polymorphism and vitamin D levels in patients with ulcerative colitis

作　　者：郑波[1] 裴继华[1] 王建嶂[1] 曹曙光[1] 

机构地区：[1]温州医科大学附属第二医院消化内科,325000

出　　处：《中华实验外科杂志》2016年第1期221-224,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨维生素D受体（VDR）FokI基因多态性及血清25-羟维生素D[25（OH）D]水平与溃疡性结肠炎（UC）的关系。方法收集178例UC患者和202例对照者，采用直接测序法检测FokI基因多态性，同时采用酶联免疫吸附试验法检测25（OH）D水平。结果UC组中FokI位点的等位基因（C）和基因型（TC＋CC）突变频率显著低于对照组（49．4％比59．4％，P〈0．01；74．7％比84．2％，P〈0．05）。与对照组比较，UC组中不仅25（OH）D平均水平显著降低[（21．68±8．02）μg/L比（24．45±8．67）μg/L，P〈0．01]，而且25（OH）D充足（≥30μg／L）的频率亦显著降低（16．3％比25．3％，P〈0．05）。线性回归分析可见UC组中25（OH）D平均水平分别与患者的疾病严重程度（β=-0．174，P〈0．05）、C反应蛋白（β=-0．137，P〈0．01）及血小板计数（β=-0．014，P〈0．05）呈负相关。结论VDR（FokI）基因突变能降低UC的发病风险；25（OH）D平均水平低下不仅是UC患者的普遍现象，还可能影响患者的疾病严重程度。Objective To explore the association of vitamin D receptor （VDR） FokI gene polymorphism and serum 25 -hydroxyvitamin D [ 25 （OH）D] levels with ulcerative colitis （UC）. Methods After collecting 178 UC patients and 202 controls, we genotyped FokI polymorphism by a direct sequencing technique. Moreover, serum 25 （OH） D level was measured by an enzyme linked immunosorbent assay. Results The mutant allele （C） and genotype （TC ＋ CC） of FokI were less prevalent in UC patients than in contrels （49.4% vs. 59.4%,P〈0.01; 74.7% vs. 84.2%,P〈0.05, respectively）. The average 25（OH）D level was significantly lower in UC patients than in controls [ （21.68 ± 8.02） μg/L vs. （24. 45 ± 8.67） μg/L,P 〈 0. 01 ]. Moreover, the proportion of 25 （OH） D sufficiency （≥ 30 μg/L） in UC patients was significantly decreased compared to controls （16. 3% vs. 25.3% ,P 〈 0.05 ）. Linear regression analysis revealed that the average 25 （OH）D level in UC patients was independently and negatively correlated with the severity of UC （ β = -0. 174, P 〈 0. 05 ）, C - reactive protein （ β = -0. 137, P 〈 0. 01 ） and platelet count （ β = - 0. 014, P 〈0. 05 ） respectively. Conclusion Our findings suggest that the genetic mutation of VDR（FokI） engenders a lower risk of UC. Lower 25 （OH）D levels might not only be a common phenomenon in UC patients, but also might affect the severity of UC.

关 键 词：维生素D 基因多态性 溃疡性结肠炎 

分 类 号：R681[医药卫生—骨科学]