参附注射液对脓毒症大鼠心肌组织Bcl-2、Bax表达的影响  

Effect of Shenfu Injection on the Expression of Bcl-2 and Bax in Rats with Sepsis

作　　者：胡丹丹[1] 徐慧连[1] 楼黎明[1] 

机构地区：[1]浙江中医药大学附属第三医院呼吸科,杭州310005

出　　处：《浙江中西医结合杂志》2016年第1期14-17,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2009CA041);浙江省自然科学基金(No.LY12H15004)

摘　　要：目的研究参附注射对脓毒症大鼠心肌细胞凋亡及Bcl-2、Bax蛋白表达的影响,阐明参附注射液抗脓毒症心肌损伤的相关机制。方法雄性SD大鼠随机分为假手术组、模型组和参附组,每组8只。以盲肠结扎穿孔(cecal ligation and puncture,CLP)法建立脓毒症大鼠动物模型,观察各组大鼠心肌酶谱变化;凋亡原位末端标记法(TUNEL)检测心肌细胞凋亡指数,RT-PCR法检测心肌细胞Bcl-2 mRNA、Bax mRNA表达。结果与假手术组比较,模型组、参附组心肌肌酸激酶同工酶(CK-MB)、肌酸激酶(CK)均明显升高,差异有统计学意义[CK-MB:(4208.51±382.57)U/L、(3584.59±347.06)U/L比(2187.41±556.63)U/L,P<0.05;CK:(3216.75±565.42)U/L、(1986.21±366.73)U/L比(1189.40±245.87)U/L,P<0.05];与参附组比较,模型组心肌酶明显升高,差异有统计学意义(P<0.05)。三组均可见大鼠心肌细胞凋亡,与假手术组比较,模型组、参附组TUNEL平均光密度(MOD)升高,差异有统计学意义(0.24±0.11、0.18±0.09比0.09±0.05,P<0.05);模型组升高最明显,与参附组比较差异有统计学意义(P<0.05)。模型组、参附组大鼠心肌细胞Bcl-2 mRNA表达均较假手术组降低,差异有统计学意义[(3.83±0.07)×10^(-3)、(4.86±0.08)×10^(-3)比(5.75±0.09)×10^(-3),P<0.05];参附组Bcl-2 mRNA表达高于模型组(P<0.05)。模型组、参附组Bax mRNA表达均较假手术组升高[(7.12±0.08)×10^(-3)、(6.26±0.06)×10^(-3)比(4.61±0.05)×10^(-3),P<0.05];参附组Bax mRNA表达低于模型组(P<0.05)。结论参附注射液能减轻脓毒症大鼠的心肌损伤,其作用机制与上调Bcl-2 mRNA的表达,下调Bax mRNA表达,减少心肌细胞凋亡相关。Objective To investigate the effect of Shenfu injection on the expression of BCL-2 and Bax and myocardial cellapoptosis in rat models with sepsis, in order to elucidate the anti-myocardial injurymechanism of Shengfu Injection. Methods Twenty-fourmale SD rats were randomly divided 3 groups： sham-operated group（n=8）,model group（n=8） and Shenfu group（n=8）. Rat sepsis models were established through cecal ligation and puncture.Myocardial creatine kinase（CK） and itsisozyme（CK-MB） levels in plasma were detected in 24 h after sepsis, myocardial apoptosis were detected by TUNEL（Td T d UTP nick and labelling） technique,and the expression of Bcl-2 mRNA and Bax mRNA in myocardial cells were detected by RT-PCR. Results Compared with sham-operated group, CK and CK-MB levels in model group and Shenfu group were higher with a significant difference（CK-MB：4208.51 ±382.57U/L, 3584.59 ±347.06U/L vs 2187.41 ±556.63U/L; CK： 3216.75 ±565.42U/L, 1986.21 ±366.73U/L vs1189.40 ±245.87U/L; all P 0.05）; CK and CK-MB in model group were higher than those in Shenfu group（P 0.05）. Myocardial cellapoptosis was observed in 3 groups; the mean optical density in model group and Shengfu group was higher than that in sham-operated group（0.24±0.11, 0.18±0.09 vs 0.09±0.05, P〈0.05）and that in Shenfu group was lower than that in model group（P〈0.05）. The expression of Bcl-2 m RNA in myocardial cells in model group and Shenfu group was lower than that in sham-operated group[（3.83±0.07）×10^-3,（4.86±0.08）×10^-3 vs（5.75±0.09）×10^-3, P〈0.05]; that in Shenfu group was higher than that in model group（P〈0.05）. The expressionof Bax m RNA in myocardial cells in model groupand Shenfu group was higher than that in sham-operated group[（7.12±0.08）×10^-3,（6.26±0.06）×10^-3 vs（4.61±0.05）×10^-3, P〈0.05]; that in Shenfu group was lower than that in model group（P 0.05）. Conclusion Shengfu injection canalleviatesepsis-induced myocardial injury in rats through increas

关 键 词：大鼠 脓毒症 心肌 BCL-2 BAX 参附注射液 

分 类 号：R285.5[医药卫生—中药学]