中华蜜蜂与意大利蜜蜂性成熟处女蜂王蛋白质组比较分析  

Proteomic Comparison of Sexual Matured Queen between Apis cerana cerana and Apis mellifera ligustica

作　　者：吴小波[1] 王子龙[1] 李淑云[1] 颜伟玉[1] 曾志将[1] 

机构地区：[1]江西农业大学蜜蜂研究所,江西南昌330045

出　　处：《江西农业大学学报》2015年第6期1057-1062,1075,共7页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家蜂产业技术体系资助项目(No.CARS-45-kxj12);国家自然科学基金项目(31060327);江西省科技支撑计划项目(20141BBF60033);江西省教育厅科技计划项目(GJJ13280)

摘　　要：为了比较分析中华蜜蜂和意大利蜜蜂两蜂种处女蜂王性成熟期蛋白表达差异。试验采用双向电泳法建立中华蜜蜂和意大利蜜蜂处女蜂王性成熟期蛋白质表达谱,通过质谱分析与数据库检索,鉴定部分差异蛋白。研究发现:在中华蜜蜂蜂王和意大利蜜蜂蜂王中分别检测到2 205、2 417个蛋白点,两者之间的差异表达蛋白点有168个。其中,在中华蜜蜂蜂王高度表达的蛋白点有90个;在意大利蜜蜂蜂王中高度表达的蛋白点有78个。对部分差异蛋白进行质谱分析,共鉴定了19个蛋白点,其中在中华蜜蜂性成熟处女蜂王中上调表达的蛋白有副肌球蛋白、肌钙蛋白T、ATP合成酶以及丙酮酸脱氢酶等;在意大利蜜蜂性成熟处女蜂王中上调表达的蛋白有NADH辅酶Q氧化还原酶、保幼激素酸甲基转移酶、肌钙蛋白以及气味结合蛋白19前体等。中华蜜蜂与意大利蜜蜂性成熟处女蜂王体内存在大量蛋白表达差异,这些差异表达的蛋白质可能与两蜂种蜜蜂行为生物学有关。This study aims to investigate the differentially expressed proteins of sexual matured queens between Apis cerana cerana and Apis mellifera ligustica by comparison of proteome profiles.Two-dimensional gel electrophoresis（ 2-DE） was used to establish the proteomic maps of the two species of sexual matured queens.Part of differentially expressed proteins were identified by mass spectrometry and database search. The results showed that 2 205 and 2 417 proteins spots were detected respectively in the sexual matured queens of Apis cerana cerana and Apis mellifera ligustica,and 168 differentially expressed protein spots were found. 90 proteins were significantly up-expressed in the sexual matured queens of Apis cerana cerana while 78 proteins were significantly up-expressed in the sexual matured queens of Apis mellifera ligustica.19 differentially expressed proteins were identified by mass spectrometry.The Paramyosin,Actin,ATP synthase and Pyruvate dehydrogenase were up-expressed in the queens Apis cerana cerana,while the NADH-ubiquinone oxidoreductase,Juvenile hormone acid methyltransferase,Troponin T and Odorant binding protein 19 precursor were up-expressed in the queens Apis mellifera ligustica.A large number of proteins in queens between Apis cerana cerana and Apis mellifera ligustica showed expression changes,which might be related to the difference in behaviour biology of the two kinds of queens.

关 键 词：中华蜜蜂 意大利蜜蜂 蜂王 蛋白质组 

分 类 号：S893.2[农业科学—特种经济动物饲养] S893.3[农业科学—畜牧兽医]