尼奥罗非鱼及其亲本中性别分化相关基因的表达模式比较  

Comparison of expression patterns of sexual differentiation related genes between tilapia Oreochromis niloticus,O.aureus and their hybrids

作　　者：伍勇[1] 简伟业 赵永华[1] 王映[1] Thammaratsuntorn Jeerawat 罗明坤[1] 赵金良[1] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [2]茂名伟业罗非鱼良种场,广东茂名525000

出　　处：《大连海洋大学学报》2015年第6期580-584,共5页Journal of Dalian Ocean University

基　　金：茂名市重大科技专项(2012A01006);现代农业产业技术体系专项(CARS-49-4B);上海高校知识服务平台项目(ZF1206)

摘　　要：Dmrt1、Amh、Cyp19a1a、Foxl2是鱼类性别决定与分化相关的重要基因,为了研究罗非鱼种间杂交后代的性别形成机制,对尼奥罗非鱼Oreochromis niloticus(尼罗罗非鱼Oreochromis niloticus♀×奥利亚罗非鱼Oreochromis aureus♂)及其亲本中这4个性别分化相关基因的性别表达模式进行了比较分析。结果表明:Dmrt1、Amh基因在尼罗罗非鱼、奥利亚罗非鱼精巢中的表达量显著或极显著高于卵巢(P<0.05或P<0.01),而Cyp19a1a、Foxl2基因在卵巢中的表达量则显著高于精巢(P<0.05),4个基因在尼奥罗非鱼精巢、卵巢中的表达模式与其亲本基本一致;奥利亚罗非鱼精巢中Dmrt1基因表达高于尼罗罗非鱼,卵巢中Cyp19a1a、Foxl2基因表达也高于尼罗罗非鱼;尼奥罗非鱼精巢、卵巢中4个基因的表达水平均低于其亲本,特别是精巢中Cyp19a1a、Foxl2基因的表达水平极低。推测Cyp19a1a表达抑制可能是引起尼奥罗非鱼雄性率高的重要条件之一。The gene expression patterns of Dmrt1,Amh,Cyp19a1a and Foxl2 involved in multiple sex determination in fish were compared between the male and female in tilapia Oreochromis niloticus,O.aureus and their hybrids（O.niloticus♀ × O.aureus♂） to research for sexual differentiation mechanim.The sexual differential expression levels of Dmrt1and Amh in tilapia O.niloticus and O.aureus were significantly or very significantly higher in testis than that in ovary（P 〈 0.05 or P 〈 0.01）,while Cyp19a1a and Foxl2 were expressed significantly higher in ovary than that in the testis（P 〈 0.05）.Sexual expression pattern of these genes in gonads of O.niloticus ♀ × O.aureus♂ was similar to that in their parents.There was higher expression of Dmrt1 in testis of O.aureus than that in O.niloticus and there was higher expression of Cyp19a1a and Foxl2 in ovary of O.aureus than that in O.niloticus.There were lower expression levels of the four genes in the gonads of O.niloticus × O.aureus than those in their parents in some degrees.It is speculated that inhibition of Cyp19a1a expression may be involved in high male rate of the tilapia hybrid offsprings.

关 键 词：罗非鱼 性别分化相关基因 基因表达 

分 类 号：S917.4[农业科学—水产科学]