富血小板血浆对大鼠海绵体神经损伤的修复效应  

The therapeutic effect of platelet rich plasma in a rat model of cavernous nerve injury

作　　者：丁协刚[1] 李世文[1] 郑新民[1] 胡万里[1] 罗仪[1] 王行环[1] 

机构地区：[1]武汉大学中南医院泌尿外科,湖北武汉430071

出　　处：《现代泌尿外科杂志》2016年第1期59-62,共4页Journal of Modern Urology

摘　　要：目的探讨富血小板血浆对大鼠海绵体神经损伤的修复效应。方法 24只SD雄性大鼠随机分成假手术组、双侧海绵体神经钳夹损伤组及损伤后富血小板血浆治疗组,术后3个月通过电刺激观察海绵体内压的变化来反映其勃起功能,随后取海绵体神经干进行甲苯胺蓝染色观察有髓鞘轴突的数目变化及阴茎组织行烟酰胺嘌呤二核苷酸磷酸(NADPH)染色检测一氧化氮合酶(NOS)阳性神经纤维数目来反映神经再生情况。结果术后3个月,损伤组最大海绵体内压为(32.7±12.2)cmH2O,明显低于假手术组(108.9±12.8)cmH2O(P<0.01);而富血小板血浆治疗组为(83.1±12.9)cmH2O,明显高于损伤组(P<0.01)。甲苯胺蓝染色损伤组的有髓鞘轴突个数为(60.3±14.5)个,明显低于假手术组(180.9±20.7)个,(P<0.01),而富血小板血浆治疗组为(114.9±23.9)个,明显高于损伤组(P<0.01)。阴茎背神经NOS阳性神经纤维数目,治疗组为(151.8±20.3)个与假手术组(285.6±57.3)个和损伤组(77.5±16.6)个相比,差异均有统计学意义(P<0.05)。结论局部应用富血小板血浆能明显促进受损海绵体神经的神经修复和勃起功能的恢复。Objective To investigate the effect of platelet rich plasma （PRP） on the recovery in a rat model of cavernous nerve injury. Methods A total of 24 Sprague-Dawley male adult rats were equally randomized into 3 groups, receiving sham operation, bilateral cavernous nerve crushing with no further intervention, and bilateral cavernous nerve crushing with an immediate application of PRP to the site of cavernous nerve crush injury, respectively. Erectile function was assessed by cavernosal nerve electrostimulation after 3 months and nerve regeneration was assessed by toluidine blue staining of CN and nicotinam. ide adenine dinucleotide phosphate （NADPH）-diaphorase staining of penile tissue. Results The mean maximal intracavern- ous pressure （ICP） in the PRP treated group （83.1 ±12.9）cmH20 was significantly higher than in injured control group （32. 7±12.2, P〈0. 01）, but lower than in sham group （108.9± 12.8, P〈0. 01）. The PRP treated group had more myelinated axons of CNs （114.9±23.9） than the injured control group （60.3± 14.5, P〈0. 01）, but fewer than the sham group （180.9：k 20.7, P〈0.01）. Furthermore, the PRP treated group had more NOS diaphorase positive nerve （151.8±20.3） than the injured control group （77.5±16.6, P〈0.05）, but fewer than the sham group （285.6±57.3, P〈0.05）. Conclusion The application of PRP to the site of CN-crush injury facilitates nerve regeneration and recovery of erectile function.

关 键 词：富血小板血浆 海绵体神经 勃起功能障碍 

分 类 号：R698[医药卫生—泌尿科学]