基于cpDNA片段探讨中-日间断分布双花木属植物的系统发育学  

An Analysis of Phylogenetic Relationship of the Genus Disanthus Distributed Disjunctively in China and Japan Based on cp DNA Sequences

作　　者：李丽卡 李象钦 谢国文[1] 李海生[2] 郑毅胜[1] 藤婕华 高锦伟[1] 

机构地区：[1]广州大学生命科学学院,广州510006 [2]广东第二师范学院生物与食品工程学院,广州510303

出　　处：《生物技术通报》2016年第1期80-87,共8页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(31270259;30970191;30470146)

摘　　要：双花木属(Disanthus)植物为东亚特有濒危物种和典型的中-日间断分布成分,基于叶绿体DNA(cp DNA)的3个片段(psb A-trn H、rps16F-R2、psa A-ycf3)联合对双花木属16个居群的164个个体进行系统发育学研究,系统发育的结果支持日本的D.Cercidifolius和中国的D.cercidifolius subsp.Longipes为单系类群,两个群体间无共享单倍型,且结果进一步支持日本群体分为两个地理组,一个来自日本本州岛的中部(居群QF,CY),一个是来自日本本州岛的西南部(居群gz、gd和JH)。PERMUT分析结果表明,双花木属居群间总的遗传多样性Ht(0.725)高于居群内平均遗传多样性场Hs(0.148),居群遗传差异Nst(0.905)大于Gst(0.796),存在极显著的谱系地理学结构。AMOVA进一步表明,双花木属的变异主要来自群体间,即中国和日本的两个群体分化明显,变异占53.08%,组内种群间的变异占24.11%,来自个体间的变异为22.80%。Disanthus is an endemic and endangered genus in East Asia and distributed disjunctively in China and Japan. In this project, we conducted the phylogenetic study of 164 individual plants in 16 natural populations of Disanthus based on the 3 fragments（psb A-trn H, rps16F-R2, and psa A-ycf3）of chloroplast DNA（cp DNA）. The results of the phylogenetic relationship confirmed that Disanthus gercidifoliusin Japan and Disanthus cercidifolius subsp. Longipes in China were monophyletic groups, and they had no share of haploid type. Furthermore, Japanese populations could be divided into 2 geographical branches, one from the center of Honshu（population QE and CY）, and the other from the southwestern Honshu（population ga, gd and JH）. PERMUT analysis showed that total genetic diversity（Ht = 0.725）of Disanthusbetween populations was higher than the average genetic diversity（Hs = 0.148）within populations. Regarding the genetic difference between populations, Nst（0.905）was larger than Gst（0.976;P〈 0.01）, demonstrating that there were significantly geographical structure of pedigree. AMOVA results further revealed that genetic variation occurred mainly between populations（53.08%）, while 24.11% among populations within a group and 22.80% among individuals within a population. These results indicated that the genetic differentiation within Disanthus between Chinese and Japanese subspecies were obvious.

关 键 词：双花木属 濒危 系统发育 

分 类 号：Q949[生物学—植物学]