藏鸡致病性沙门菌的耐药性分析及质粒耐药基因的检测  

Analysis of antimicrobial resistance and detection of plasmid-mediated resistance genes in pathogenic Salmonella isolated from Tibetan chickens

作　　者：杨丹[1] 贺安祥[1,2] 邓俊良[1] 蒋忠荣[2] 冯卫东[2] 徐志文[1] 李平[2] 任志华[1] 邓世金 衡久红 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室环境公害与动物疾病四川省高校重点实验室,四川成都611130 [2]甘孜州畜牧业科学研究所,四川康定626000

出　　处：《中国兽医科学》2016年第1期84-90,共7页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848);甘孜州2013年度应用研究与开发科技项目(2013103);四川农业大学双支计划项目(3075103)

摘　　要：为研究藏鸡致病性沙门菌的耐药性及其质粒耐药基因携带情况,分别采用琼脂纸片扩散法和PCR扩增技术对14株藏鸡致病性沙门菌进行耐药表型及质粒耐药基因检测,并通过质粒接合试验验证其质粒耐药基因的水平传播。结果显示,14株分离菌对氨苄西林的耐药性(85.71%)最高,对阿莫西林(78.57%)、羧苄西林(78.57%)、复方新诺明(71.43%)、利福平(71.43%)、四环素(64.29%)的耐药比较严重,仅对头孢唑啉、庆大霉素、丁胺卡那敏感,且92.86%(13/14)的菌株表现为多重耐药性。此外,14株菌共检出blaTEM(78.57%)、blaCTX-M(71.43%)、blaOXA(42.86%)、blaPSE(50.00%)、blaDHA(14.28%)、blaCMY-2(50.00%)、qnrA(28.57%)、qnrS(42.86%)、aac(6′)-Ib-cr(35.71%)、oqxA(42.86%)和oqxB(35.71%)等11种质粒耐药基因。质粒耐药基因可通过接合试验水平转移,其接合成功率为57.14%(8/14)。结果表明,这14株藏鸡致病性沙门菌的耐药性比较严重,且广泛携带质粒耐药基因。To investigate the characteristics of antimicrobial resistance and the prevalence of plasmidmediated resistance genes in 14 pathogenic Salmonellaisolates from Tibetan chickens,the resistant phenotypes and plasmid-mediated resistance genes were detected in 14 isolates by Kirby-Bauer method and PCRsequencing.Conjugation experiments were performed among plasmid-mediated resistance genes.The results indicated that the highest prevalence of resistance was to ampicillin（85.71%）,followed by amoxicillin（78.57%）,carbenicillin（78.57%）,sulphame-thoxazole（71.43%）,rifampicin（71.43%）,and tetracycline（64.29%）,but all isolates were sensitive to cefazolin,gentamicin,and amikacin.Total 92.86%（13/14）of the isolates were multidrug resistance.Additionally,the plasmid-mediated resistance genes blaTEM,blaCTX-M,blaOXA,blaPSE,blaDHA,blaCMY-2,qnrA,qnrS,aac（6′）-Ib-cr,oqxAand oqxB were found in 78.57%,71.43%,42.86%,50.00%,14.28%,50.00%,28.57%,42.86%,35.71%,42.86% and35.71% of 14 isolates,respectively.The plasmid-mediated resistance genes were transferred from 57.14%（8/14）of the Salmonellaisolates into the E.coli J53 by conjugation experiments.Our findings showed that the Salmonellaisolated from Tibetan chickens were serious resistance to antimicrobial agents,and plasmid-mediated resistance genes were widely existed.

关 键 词：藏鸡 沙门菌 耐药性 质粒耐药基因 质粒接合 

分 类 号：S852.612[农业科学—基础兽医学]