高原甘加型藏羊发情周期不同阶段卵巢FSHR和LHR基因表达差异的研究  

Expression of FSHR gene and LHR gene in the ovary of Ganjia Tibetan sheep in different stages of estrous cycle

作　　者：王文芳[1] 何玉琴[1] 韩志磊[1] 史军红[1] 火静萍 朱雪雁 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《中国兽医科学》2016年第1期97-103,共7页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31360595)

摘　　要：为研究高原甘加型藏羊发情周期卵巢组织中促卵泡素受体(FSHR)和促黄体素受体(LHR)基因在mRNA水平上的表达规律,选取发情周期不同阶段的甘加型藏羊24只,从其卵巢组织中提取总RNA,采用实时荧光定量PCR(RT-PCR)技术,以GAPDH为内参基因,比较研究了高原甘加型藏羊发情周期卵巢组织中FSHR和LHR基因在mRNA水平的表达差异。结果显示,FSHR mRNA和LHR mRNA在整个发情周期的卵巢组织中都有表达。发情前期、发情期、发情后期、间情期的FSHR mRNA表达量分别为46.0±0.21、51.7±0.33、17.7±0.56、1.0±0.82,其中发情期表达量最高,极显著高于间情期(P<0.01);LHR mRNA表达量分别为19.8±2.63、30.1±1.81、85.3±0.63、1.0±1.37,其中发情后期表达量最高,极显著高于间情期(P<0.01)。甘加型藏羊FSHR和LHR发情周期在mRNA水平上表达量不相同,呈现一种动态变化趋势。这一研究结果为藏羊的生殖内分泌调控机制研究提供了分子水平的试验数据和进一步研究依据。The objective of this experiment is to examine the mRNA levels of FSHR gene and LHR gene in ovaries of estrous cycle in Ganjia Tibetan sheep.The 24 sheep in different stages of estrous cycle were killed for ovaries and real time fluorescence quantitative PCR（RT-PCR）method with GAPDH as reference gene was established.The results showed that the FSHR gene and LHR gene were expressed in the ovaries of Ganjia Tibetan sheep during the whole estrous cycle.The relative expression of FSHR in proestrus,estrus,metestrus and diestrus were respectively 46.0±0.21,51.7±0.33,17.7±0.56and1.0±0.82.The FSHR mRNA relative expression in estrus was the highest and was very significantly different from that in diestrus（P〈0.01）.The relative expression of LHR in proestrus,estrus,metestrus and diestrus were respectively 19.8±2.63,30.1±1.81,85.3±0.63 and 1.0±1.37.The LHR mRNA relative expression in metestrus was the highest and was very significantly different from that in diestrus（P〈0.01）.The relative expression of FSHR gene and LHR gene in mRNA levels in Ganjia Tibetan sheep were different and presented a dynamic trend.The study is provided the molecular experiment data and basis for further study for reproductive endocrine regulation mechanism of Tibetan sheep.

关 键 词：高原甘加型藏羊 卵巢 促卵泡素受体mRNA 促黄体素受体mRNA 发情周期 实时荧光定量PCR 

分 类 号：S852.2[农业科学—基础兽医学]