环介导等温扩增法检测转基因玉米NK603  

Loop-mediated isothermal amplification method for detection of transgenic maize NK603

作　　者：杨丽霞[1] 

机构地区：[1]长沙市食品质量安全监督检测中心,湖南长沙410013

出　　处：《食品与机械》2016年第1期56-58,80,共4页Food and Machinery

基　　金：湖南省科技计划重点项目(编号:2013WK2003)

摘　　要：针对转基因玉米的特异基因(GenBank accession number AX342369)设计引物,建立转基因玉米NK603品系特异性基因环介导等温扩增方法。对该方法进行特异性、灵敏度分析。结果表明:LAMP法检测转基因玉米NK603品系成分特异性强、灵敏度高、定性检出限为0.1%。该方法快速、简便,可在作物转基因筛查中推广使用。The specific LAMP primers of genetically modified maize NK603 were designed on the basis of the published sequence （GenBank accession number AX342369）. Established the specific loopmediated isothermal amplification （LAMP） method for the detection of the transgenic maize line NK603 strains. The specificity and sensitivity of this method were evaluated. The results showed that LAMP could detect the transgenic maize NK603 from several GM events specifically and effectively with LOD of 0. 1%. Since this method is rapid, simple, it could be applied in GMO detection.

关 键 词：转基因 玉米 NK603品系 环介导等温扩增 

分 类 号：S513[农业科学—作物学]