水通道蛋白1过表达对小鼠施万细胞形态及水转运的影响  

Effects of AQP1 overexpression on morphology and water transport in mouse Schwann cells

作　　者：章杰[1] 杨红华[1] 彭丽[1] 江华[2] 

机构地区：[1]南昌大学第一附属医院整形外科,330006 [2]第二军医大学上海长征医院整形外科

出　　处：《中华整形外科杂志》2016年第1期60-64,共5页Chinese Journal of Plastic Surgery

基　　金：National Natural Science Foundation of China (31271264)国家自然科学基金

摘　　要：目的探讨水通道蛋白1（AQP1）基因表达和施万细胞肿胀之间的关系，明确AQP1在面神经损伤后水肿中的功能。方法细胞免疫荧光双标法验证小鼠面神经来源的原代施万细胞AQP1表达；应用慢病毒介导的过表达技术上调施万细胞AQP1表达；Lenti—AQP1感染施万细胞后，每天在倒置相差显微镜下观察细胞形态变化，并与CTRL组（emptyvector）比较，连续6d；细胞容积测定量化Lenti—AQP1细胞与CTRL细胞水含量变化。结果小鼠面神经来源的原代施万细胞表达AQP1；构建的小鼠Lenti—AQP1慢病毒载体感染施万细胞后，能在mRNA和蛋白水平明显增加AQP1表达（P〈0．05）；AQP1过表达能使施万细胞肿胀，细胞形态近似圆形，细胞水含量显著增加（P〈0．01）。结论AQP1是控制施万细胞快速水转运的主要因素，对面神经水肿的发生有重要作用。Objective To determine the effect of AQP1 gene on facial nerve edema following injury through investigation of the relationship between the expression of AQP1 gene and Schwann cells swelling. Methods The AQP1 expression in Schwann cells of mouse facial nerve tissues was detected by immunofluorescent staining. The transgenic protocol by lentivirus transduction was used to specifically up- regulate AQP1 expression in Schwann cells. Lenti-AQP1 and CTRL （empty vector）transduced ceils were observed during gene overexpression every 24 h for 6 days by using phase contrast microscopy. Cell volume of CTRL and Lenti-AQP1 treated cells was measured daily from the day of treatment, through day 6. Results Schwann cell primary cuhures maintained a high level of AQP1 water channels, representing an ideal cell model to study the role of AQP1 in the facial nerve. The expression of AQP1 mRNA and protein in Schwann ceils infected with the Lenti-AQPl was increased significantly compared with CTRL lentivirus （P 〈 0. 05）. Lenti-AQP1 caused cell swelling in cultured Schwann cells, as validated by cell volume determinations （P 〈 0. 01 ）. Conclusions AQP1 is an important factor responsible for the fast water transport of cultured Schwann cells. It plays an important role in facial nerve edema.

关 键 词：水通道蛋白1 施万细胞 面神经损伤 基因治疗 

分 类 号：R745[医药卫生—神经病学与精神病学]