硫化氢参与镉诱导拟南芥气孔的关闭  

Hydrogen Sulfide Mediates Cd^(2+)-induced Stomatal Closure in Arabidopsis thaliana

作　　者：乔增杰[1] 王婷[1] 金竹萍[1] 刘志强[1] 裴雁曦[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2016年第1期146-151,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31400237);山西大学科研训练项目(2014012135);山西省青年科技基金(2014021026-2;2014021026-1)

摘　　要：以拟南芥为实验材料,利用分光光度法、显微观测法及药理学实验,研究了镉(Cd^(2+))、硫化氢(H2S)和脱落酸(ABA)处理对拟南芥野生型(wild type,WT)、abi5和lcd气孔运动的影响,以及H_2S合成酶L-半胱氨酸脱巯基酶(LCD)的活性和体内H2S含量的变化。结果表明:光照条件下,随着Cd^(2+)浓度的增加,野生型拟南芥气孔孔径逐渐减小,LCD的活性及内源H_2S含量依次升高。200μmol/L的CdCl_2可以减小abi5的气孔开度,且50μmol/L的H_2S也可以减小abi5和WT的气孔开度,而ABA并不能减小abi5的气孔开度,说明Cd^(2+)诱导的气孔关闭主要依赖H2S信号。实验进一步验证了这种现象:与WT相比,200μmol/L的CdCl_2对LCD缺失突变体(lcd)气孔开度的影响不大,加入H2S的供体NaHS后,lcd气孔关闭。研究发现,Cd^(2+)可通过增强LCD的活性,促进L-半胱氨酸分解生成H_2S。Through pharmacological combined with spectrophotography, we study the effects of Cd^2＋ , ABA and HzS on stomatal closure, L-cysteine desulfhydrase （LCD） activity and H2S generation in Arabidopsis thaliana. The results showed that as the concentration of Cd^2＋ increased under light condition, the stomatal closure was gradually increased in Arabidopsis. Cd^2＋ treatment increased LCD activity and induced Hz S generation. The stomatal closure was induced by 200 μmol/L CdCl2 or 50 μmol/L H2S in abi5, but ABA was not inoperative. The mutant lcd reduced Hz S generation and inhibited Cd^2＋-induced the stomatal closure compared with the WT. The results suggested that the accumulation of H2S via enhancing the LCD activity could finally induced the stomatal closure of Arabidopsis.

关 键 词：拟南芥 硫化氢 Cd^2+ 气孔关闭 

分 类 号：Q494[生物学—生理学]